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Nanyang Technological UniversityFluorescence Labeling to Visualize Low-Expressed Proteins in Zebrafish
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•
09:38 min
•
January 24th, 2025
DOI :
January 24th, 2025
•Xuepu Jin*1, Binghuang Zhang*1, Yu Sun*1, Yahan Duan1, Junchen Lu1, Jiannan Liu2, Jiahuai Han1,3,4, Yingying Zhang1
1State Key Laboratory of Cellular Stress Biology, School of Life Sciences, Faculty of Medicine and Life Sciences, Xiamen University, 2Technology Center, Shanxi Xinghuacun Fenjiu Distillery Co., Ltd., 3Research Unit of Cellular Stress of CAMS, Cancer Research Center of Xiamen University, Xiang'an Hospital of Xiamen University, School of Medicine, Xiamen University, 4Laboratory Animal Center, Xiamen University
Chapters in this video
0:00
Introduction
1:19
Efficiency Testing of sgRNAs in Zebrafish via Microinjection and Genotyping
5:27
Donor Plasmid Construction and Microinjection for Fluorescent Protein Tagging in Zebrafish
9:01
Representative Results
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ISSN 2578-6326
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