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Nanyang Technological University

تنقية مجمعات الأصلية للدراسة الهيكلية باستخدام طريقة العلامة جنبا الى جنب الانجذاب

The Tandem Affinity Purification (TAP) method has been used extensively to isolate native complexes from cellular extract, primarily eukaryotic, for proteomics. Here, we present a TAP method protocol optimized for purification of native complexes for structural studies.

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Chapters in this video

0:05

Title

0:38

Harvesting S. cerevisiae Cells

1:44

Lyse Cells and Lysate Clarification

4:43

Resin Equilibration and Binding and On Column TEV Cleavage

6:47

Packing and Eluting from Calmodulin Resin

8:20

Results: TAP Purification of U1 snRNP Complex in S. cerevisiae

9:55

Conclusion

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