JoVE Journal
Biology
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Nanyang Technological University9.5K Views
•
10:36 min
•
July 27th, 2016
DOI :
July 27th, 2016
•0:05
Title
0:38
Harvesting S. cerevisiae Cells
1:44
Lyse Cells and Lysate Clarification
4:43
Resin Equilibration and Binding and On Column TEV Cleavage
6:47
Packing and Eluting from Calmodulin Resin
8:20
Results: TAP Purification of U1 snRNP Complex in S. cerevisiae
9:55
Conclusion
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