Nitroreductase/Metronidazole-Mediated Ablation and a MATLAB Platform (RpEGEN) for Studying Regeneration of the Zebrafish Retinal Pigment Epithelium
2.5K Views
•
13:12 min
•
March 2nd, 2022
DOI :
March 2nd, 2022
•Chapters in this video
0:05
Introduction
1:00
Screening Zebrafish Larvae for rpe65a:nfsB-eGFP and Genetic Ablation of the Retinal Pigment Epithelium
3:02
Incorporating Pharmacological Treatment into Zebrafish Retinal Pigment Epithelium Ablation Protocol
6:35
Confocal Microscope Z-stack Image Preprocessing in FIJI (ImageJ)
8:19
Quantification and Visualization of RPE Regeneration Using RpEGEN Scripts
10:42
Results: RPE Expression, Ablation, Regeneration Quantification
12:39
Conclusion
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