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Biology

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Monitoring On-Target Signaling Responses in Larval Zebrafish - Z-REX Unmasks Precise Mechanisms of Electrophilic Drugs and Metabolites

731 Views

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05:28 min

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June 2nd, 2023

DOI :

10.3791/64846-v

June 2nd, 2023

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Kuan-Ting Huang¹, Phillippe Ly¹, Jesse R. Poganik², Saba Parvez³, Marcus J. C. Long⁴, Yimon Aye¹

¹Swiss Federal Institute of Technology Lausanne (EPFL), ²Division of Genetics, Department of Medicine, Brigham and Women’s Hospital, Harvard Medical School, ³Department of Pharmacology and Toxicology, College of Pharmacy, University of Utah, ⁴University of Lausanne (UNIL)

Summary

Zebrafish targeting reactive electrophiles and oxidants (Z-REX) is a chemical biology-based method for the investigation of reactive small-molecule signaling. This technique can be applied to live fish of different developmental stages. Here, we couple standard assays in zebrafish with Z-REX for signaling pathway analysis.

Explore More Videos

Larval Zebrafish

Electrophilic Drugs

Reactive Ligands

Signaling Responses

Kinase Inhibitors

Precision Medicine

Phenotypic Assay

Injection Technique

Embryo Incubation

Fluorescent Cells

Apoptosis Analysis

Chapters in this video

0:04

Introduction

1:02

Fish Embryo Microinjection

1:45

Z‐REX Treatment

2:31

Downstream Assay

3:13

Results: Analysis of Treated and Untreated Embryos

4:28

Conclusion

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