In Vitro Assay of Plasmodium-Infected Red Blood Cell Killing by Cytotoxic Lymphocytes
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08:20 min
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August 17th, 2022
DOI :
August 17th, 2022
•Rozdziały w tym wideo
0:04
Introduction
0:58
Experimental Malaria Infection in Mouse
1:49
Obtaining Fresh Whole Mouse Splenocytes
3:30
Cytotoxic Effector Cell Purification: CD8a+ T Cell Negative Selection
4:22
P. yoelii Infected RBC Isolation
5:21
CFSE Labeling of RBCs, Preparation for Flow Cytometry and Cytotoxic Lymphocyte Coculture
6:22
Results: P. yoelii - iRBC Enrichment and Assessment of cell lysis Percentage of P. yoelii - iRBCs by CD8+ lymphocytes
7:39
Conclusion
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