Using Nanoplasmon-Enhanced Scattering and Low-Magnification Microscope Imaging to Quantify Tumor-Derived Exosomes

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7.3K Views

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09:30 min

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May 24th, 2019

DOI :

10.3791/59177-v

May 24th, 2019

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Meihua Wan¹^,², Pouya Amrollahi²^,³, Dali Sun⁴, Christopher Lyon²^,³, Tony Y. Hu²^,³

¹Department of Integrated Traditional Chinese and Western Medicine, West China Hospital of Sichuan University, ²Virginia G. Piper Biodesign Center for Personalized Diagnostics, The Biodesign Institute, Arizona State University, ³School of Biological and Health Systems Engineering, Arizona State University, ⁴Department of Electrical and Computer Engineering, North Dakota State University

Podsumowanie

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Nanoplasmon enhanced Scattering

NPES

Exosome Analysis

Tumor derived Exosomes

Clinical Settings

Biomarker Analysis

NeutrAvidin functionalized Gold Nanorods

Antibody conjugated Nanorods

PDX Mouse Models

Wet Lab Skills

Centrifugation Protocol

Capture Antibodies

Optical grade Glass Slide

Rozdziały w tym wideo

0:04

Title

1:01

Preparation of Nanoparticle Probes (AuNR-IgG)

2:17

Preparation of Extracellular Vesicle Capture Slides and Exosome Capture

5:17

Low-Magnification Dark-Field Microscope (LMDFM) Image Acquisition and Analysis

7:48

Results: Exosome Quantification by Analysis of LMDFM Images

8:25

Conclusion

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