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Chemistry

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Nanyang Technological University

Dissolution Dynamic Nuclear Polarization Instrumentation for Real-time Enzymatic Reaction Rate Measurements by NMR

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10.6K Views

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10:54 min

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February 23rd, 2016

DOI :

10.3791/53548-v

February 23rd, 2016

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Riccardo Balzan¹, Laetitia Fernandes¹, Arnaud Comment², Laetitia Pidial³, Bertrand Tavitian³, Paul R. Vasos¹

¹LCBPT - UMR8601, Institut de Chimie, Université Paris Descartes, ²Institute of Physics of Biological Systems, EPFL, ³PARCC - INSERM U970, Université Paris Descartes

Podsumowanie

The sensitivity enhancement provided by dissolution dynamic nuclear polarization (DNP) enables following metabolic processes in real time by NMR and MRI. The characteristics and performances of a dedicated dissolution DNP setup designed for study enzymatic reactions are discussed.

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Artificial Intelligence

Dynamic Nuclear Polarization

Enzymatic Reaction

Real time Measurement

Dissolution DNP

Polarizing Solution

Sample Container

Liquid Nitrogen

Rozdziały w tym wideo

0:05

Title

1:37

Polarization

3:29

Solid-state NMR Measurements

5:15

Sample Preparation and Positioning

6:19

Dissolution and Injection

7:32

Liquid-state NMR Measurement

8:25

Results: Real-time Enzymatic Reaction Rate Measurement for LDH Activity

9:08

Conclusion

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