JoVE Table of Contents: Issue 187, September 2022

An Integrated Workflow of Identification and Quantification on FDR Control-Based Untargeted Metabolome

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Journal (Biochemistry)

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Methods Collections

Experimental Quantification of Interactions Between Drug Delivery Systems and Cells <em>In Vitro</em>: A Guide for Preclinical Nanomedicine Evaluation

Experimental Quantification of Interactions Between Drug Delivery Systems and Cells In Vitro: A Guide for Preclinical Nanomedicine Evaluation

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Journal (Bioengineering)

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Methods Collections

Determining Temperature Preference of Mosquitoes and Other Ectotherms

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Journal (Biology)

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Methods Collections

Flow Cytometry Analysis of Murine Bone Marrow Hematopoietic Stem and Progenitor Cells and Stromal Niche Cells

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Journal (Biology)

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Methods Collections

Protocols for CRISPR/Cas9 Mutagenesis of the Oriental Fruit Fly <em>Bactrocera dorsalis</em>

Protocols for CRISPR/Cas9 Mutagenesis of the Oriental Fruit Fly Bactrocera dorsalis

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Journal (Biology)

Isolation of Small Preantral Follicles from the Bovine Ovary Using a Combination of Fragmentation, Homogenization, and Serial Filtration

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Journal (Biology)

Primary Culture of Porcine Retinal Pigment Epithelial Cells

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Journal (Biology)

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Methods Collections

Isolation, Characterization, and Therapeutic Application of Extracellular Vesicles from Cultured Human Mesenchymal Stem Cells

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Journal (Biology)

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Methods Collections

Quantification of Circular RNAs Using Digital Droplet PCR

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Journal (Biology)

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Methods Collections

AirID-Based Proximity Labeling for Protein-Protein Interaction in Plants

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Journal (Biology)

Visualizing Subcellular Localization of a Protein in the Heart Using Quantum Dots-Mediated Immuno-Labeling Followed by Transmission Electron Microscopy

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Journal (Biology)

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Methods Collections

A Standardized Liquid Biopsy Preanalytical Protocol for Downstream Circulating-Free DNA Applications

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Journal (Cancer Research)

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Methods Collections

An R-Based Landscape Validation of a Competing Risk Model

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Journal (Cancer Research)

Freeze-Fracture Electron Microscopy for Extracellular Vesicle Analysis

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Journal (Cancer Research)

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Methods Collections

High Spatial Resolution Chemical Imaging of Implant-Associated Infections with X-ray Excited Luminescence Chemical Imaging Through Tissue

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Journal (Chemistry)

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Methods Collections

Constructing Cyclic Peptides Using an On-Tether Sulfonium Center

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Journal (Chemistry)

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Methods Collections

Construction of Cyclic Cell-Penetrating Peptides for Enhanced Penetration of Biological Barriers

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Journal (Chemistry)

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Methods Collections

Intramyocardial Injection for the Study of Cardiac Lymphatic Function in Zebrafish

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Journal (Developmental Biology)

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Methods Collections

Investigating Long-Distance Transport of Perfluoroalkyl Acids in Wheat <em>via</em> a Split-Root Exposure Technique

Investigating Long-Distance Transport of Perfluoroalkyl Acids in Wheat via a Split-Root Exposure Technique

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Journal (Environment)

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Methods Collections

Source and Route of Pyrrolizidine Alkaloid Contamination in Tea Samples

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Journal (Environment)

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Methods Collections

<em>C. elegans</em> Gonad Dissection and Freeze Crack for Immunofluorescence and DAPI Staining

C. elegans Gonad Dissection and Freeze Crack for Immunofluorescence and DAPI Staining

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Journal (Genetics)

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Methods Collections

A Mouse Model for the Transition of <em>Streptococcus pneumoniae</em> from Colonizer to Pathogen upon Viral Co-Infection Recapitulates Age-Exacerbated Illness

A Mouse Model for the Transition of Streptococcus pneumoniae from Colonizer to Pathogen upon Viral Co-Infection Recapitulates Age-Exacerbated Illness

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Journal (Immunology and Infection)

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Methods Collections

Phage-Mediated Genetic Manipulation of the Lyme Disease Spirochete <em>Borrelia burgdorferi</em>

Phage-Mediated Genetic Manipulation of the Lyme Disease Spirochete Borrelia burgdorferi

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Journal (Immunology and Infection)

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Methods Collections

Preparation, Characteristics, Toxicity, and Efficacy Evaluation of the Nasal Self-Assembled Nanoemulsion Tumor Vaccine <em>In Vitro</em> and <em>In Vivo</em>

Preparation, Characteristics, Toxicity, and Efficacy Evaluation of the Nasal Self-Assembled Nanoemulsion Tumor Vaccine In Vitro and In Vivo

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Journal (Immunology and Infection)

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Methods Collections

A Modified Murine Heterotopic Heart Transplant Protocol Matching Contemporary Standards of Aseptic Technique, Anesthesia, and Analgesia

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Journal (Immunology and Infection)

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Methods Collections

Isolation and Quantification of Epstein-Barr Virus from the P3HR1 Cell Line

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Journal (Immunology and Infection)

Contact Hypersensitivity as a Murine Model of Allergic Contact Dermatitis

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Journal (Immunology and Infection)

Detection of Polyfunctional T Cells in Children Vaccinated with Japanese Encephalitis Vaccine <em>via</em> the Flow Cytometry Technique

Detection of Polyfunctional T Cells in Children Vaccinated with Japanese Encephalitis Vaccine via the Flow Cytometry Technique

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Journal (Immunology and Infection)

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Methods Collections

A Soluble Tetrazolium-Based Reduction Assay to Evaluate the Effect of Antibodies on <em>Candida tropicalis</em> Biofilms

A Soluble Tetrazolium-Based Reduction Assay to Evaluate the Effect of Antibodies on Candida tropicalis Biofilms

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Journal (Immunology and Infection)

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Methods Collections

Zebrafish Animal Model for the Study of Allergic Reactions in Response to Tick Saliva Biomolecules

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Journal (Immunology and Infection)

A Novel High-Throughput <em>Ex Vivo</em> Ovine Skin Wound Model for Testing Emerging Antibiotics

A Novel High-Throughput Ex Vivo Ovine Skin Wound Model for Testing Emerging Antibiotics

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Journal (Immunology and Infection)

<em>Ex Vivo</em> Analysis of Mechanically Activated Ca<sup>2+</sup> Transients in Urothelial Cells

Ex Vivo Analysis of Mechanically Activated Ca²⁺ Transients in Urothelial Cells

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Journal (Medicine)

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Methods Collections

Image Acquisition using Portable Sonography for Emergency Airway Management

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Journal (Medicine)

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Methods Collections

Infrared Thermography for the Detection of Changes in Brown Adipose Tissue Activity

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Journal (Medicine)

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Methods Collections

Bridging the Technology Divide in the COVID-19 Era: Using Virtual Outreach to Expose Middle and High School Students to Imaging Technology

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Journal (Medicine)

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Methods Collections

Modified Posterior Vertebral Column Resection for Patients with Thoracolumbar Kyphotic Deformity

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Journal (Medicine)

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Methods Collections

C-arm-Free Simultaneous OLIF51 and Percutaneous Pedicle Screw Fixation in a Single Lateral Position

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Journal (Medicine)

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Methods Collections

Electromagnetic Controlled Closed-Head Model of Mild Traumatic Brain Injury in Mice

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Journal (Neuroscience)

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Methods Collections

Imaging the Aging Cochlea with Light-Sheet Fluorescence Microscopy

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Journal (Neuroscience)

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Methods Collections

Modeling Human Cerebellar Development <em>In Vitro</em> in 2D Structure

Modeling Human Cerebellar Development In Vitro in 2D Structure

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Journal (Neuroscience)

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Methods Collections

Evaluation and Manipulation of Neural Activity Using Two-Photon Holographic Microscopy

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Journal (Neuroscience)

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Methods Collections

Fabrication and Use of Dry Macroporous Alginate Scaffolds for Viral Transduction of T Cells

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Journal (Bioengineering)

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Methods Collections

Microengineering 3D Collagen Hydrogels with Long-Range Fiber Alignment

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Journal (Bioengineering)

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Methods Collections

In Vitro Selection of Aptamers to Differentiate Infectious from Non-Infectious Viruses

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Journal (Bioengineering)

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Methods Collections

Cellular Affinity of Particle-Stabilized Emulsion to Boost Antigen Internalization

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Journal (Bioengineering)

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Methods Collections

Compost Microcosms as Microbially Diverse, Natural-like Environments for Microbiome Research in <em>Caenorhabditis elegans</em>

Compost Microcosms as Microbially Diverse, Natural-like Environments for Microbiome Research in Caenorhabditis elegans

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Journal (Biology)

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Methods Collections

Super-Resolution Microscopy of the Synaptonemal Complex Within the <em>Caenorhabditis elegans</em> Germline

Super-Resolution Microscopy of the Synaptonemal Complex Within the Caenorhabditis elegans Germline

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Journal (Biology)

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Methods Collections

CRISPR-Cas9-Mediated Precise Knock-In Edits in Zebrafish Hearts

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Journal (Biology)

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Methods Collections

Isolation of Conditionally Immortalized Mouse Glomerular Endothelial Cells with Fluorescent Mitochondria

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Journal (Biology)

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Methods Collections

Embryo Injection Technique for Gene Editing in the Black-Legged Tick, <em>Ixodes scapularis</em>

Embryo Injection Technique for Gene Editing in the Black-Legged Tick, Ixodes scapularis

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Journal (Biology)

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Methods Collections

Hand Dissection of <em>Caenorhabditis elegans</em> Intestines

Hand Dissection of Caenorhabditis elegans Intestines

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Journal (Biology)

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Methods Collections

Genetic Screen for Identification of Multicopy Suppressors in <em>Schizosaccharomyces pombe</em>

Genetic Screen for Identification of Multicopy Suppressors in Schizosaccharomyces pombe

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Journal (Biology)

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Methods Collections

Embryo Rescue Protocol for Interspecific Hybridization in Squash

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Journal (Biology)

Induction and Diverse Assessment Indicators of Experimental Autoimmune Encephalomyelitis

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Journal (Biology)

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Methods Collections

A Flow Cytometry-Based Cell Surface Protein Binding Assay for Assessing Selectivity and Specificity of an Anticancer Aptamer

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Journal (Cancer Research)

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Methods Collections

Far-Red Fluorescent Senescence-Associated β-Galactosidase Probe for Identification and Enrichment of Senescent Tumor Cells by Flow Cytometry

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Journal (Cancer Research)

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Methods Collections

Digital Spatial Profiling for Characterization of the Microenvironment in Adult-Type Diffusely Infiltrating Glioma

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Journal (Cancer Research)

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Methods Collections

Evaluation of the Spindle Assembly Checkpoint Integrity in Mouse Oocytes

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Journal (Developmental Biology)

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Methods Collections

An Efficient Clearing Protocol for the Study of Seed Development in Tomato (<em>Solanum lycopersicum</em> L.)

An Efficient Clearing Protocol for the Study of Seed Development in Tomato (Solanum lycopersicum L.)

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Journal (Developmental Biology)

Microtensiometer for Confocal Microscopy Visualization of Dynamic Interfaces

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Journal (Engineering)

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Methods Collections

Triplet Fusion Upconversion Nanocapsule Synthesis

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Journal (Engineering)

An Effective Inoculation Method for <em>Phytophthora capsici</em> on Black Pepper Plants

An Effective Inoculation Method for Phytophthora capsici on Black Pepper Plants

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Journal (Genetics)

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Methods Collections

Transient Transduction of the Strobilated Forms of <em>Echinococcus granulosus</em>

Transient Transduction of the Strobilated Forms of Echinococcus granulosus

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Journal (Genetics)

Detection of Homologous Recombination Intermediates <em>via</em> Proximity Ligation and Quantitative PCR in <em>Saccharomyces cerevisiae</em>

Detection of Homologous Recombination Intermediates via Proximity Ligation and Quantitative PCR in Saccharomyces cerevisiae

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Journal (Genetics)

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Methods Collections

A Mouse Model of Orotracheal Intubation and Ventilated Lung Ischemia Reperfusion Surgery

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Journal (Immunology and Infection)

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Methods Collections

Separation of Immune Cell Subpopulations in Peripheral Blood Samples from Children with Infectious Mononucleosis

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Journal (Immunology and Infection)

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Methods Collections

Dynamic Continuous Blood Extraction from Rat Heart <em>via</em> Noninvasive Microdialysis Technique

Dynamic Continuous Blood Extraction from Rat Heart via Noninvasive Microdialysis Technique

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Journal (Medicine)

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Methods Collections

Transdermal Measurement of Glomerular Filtration Rate in Mechanically Ventilated Piglets

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Journal (Medicine)

Laparoscopic Non-Mesh Cerclage Pectopexy for Pelvic Organ Prolapse

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Journal (Medicine)

Laser-Induced Action Potential-Like Measurements of Cardiomyocytes on Microelectrode Arrays for Increased Predictivity of Safety Pharmacology

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Journal (Medicine)

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Methods Collections

Dynamic Navigation for Dental Implant Placement

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Journal (Medicine)

A Two-Step Method for Percutaneous Transhepatic Choledochoscopic Lithotomy

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Journal (Medicine)

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Methods Collections

<em>In Vivo</em> Luminal Measurement of Distension-Evoked Urothelial ATP Release in Rodents

In Vivo Luminal Measurement of Distension-Evoked Urothelial ATP Release in Rodents

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Journal (Medicine)

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Methods Collections

The Transition to an Anterior-Based Muscle Sparing Approach Improves Early Postoperative Function but is Associated with a Learning Curve

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Journal (Medicine)

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Methods Collections

Subcellular Fractionation for the Isolation of Synaptic Components from the Murine Brain

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Journal (Neuroscience)

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Methods Collections

Through a Dog's Eyes: fMRI Decoding of Naturalistic Videos from the Dog Cortex

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Journal (Neuroscience)

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Methods Collections

Establishing a Mouse Contusion Spinal Cord Injury Model Based on a Minimally Invasive Technique

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Journal (Neuroscience)

Head Implants for the Neuroimaging of Awake, Head-Fixed Rats

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Journal (Neuroscience)

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Methods Collections

Assessment of Audio-Tactile Sensory Substitution Training in Participants with Profound Deafness Using the Event-Related Potential Technique

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Journal (Neuroscience)

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Methods Collections

Optrode Array for Simultaneous Optogenetic Modulation and Electrical Neural Recording

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Journal (Neuroscience)

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Methods Collections

Inhoudsopgave

Archiveren: Issue 187, September 2022

GA NAAR

An Integrated Workflow of Identification and Quantification on FDR Control-Based Untargeted Metabolome

Experimental Quantification of Interactions Between Drug Delivery Systems and Cells In Vitro: A Guide for Preclinical Nanomedicine Evaluation

Determining Temperature Preference of Mosquitoes and Other Ectotherms

Flow Cytometry Analysis of Murine Bone Marrow Hematopoietic Stem and Progenitor Cells and Stromal Niche Cells

Protocols for CRISPR/Cas9 Mutagenesis of the Oriental Fruit Fly Bactrocera dorsalis

Isolation of Small Preantral Follicles from the Bovine Ovary Using a Combination of Fragmentation, Homogenization, and Serial Filtration

Primary Culture of Porcine Retinal Pigment Epithelial Cells

Isolation, Characterization, and Therapeutic Application of Extracellular Vesicles from Cultured Human Mesenchymal Stem Cells

Quantification of Circular RNAs Using Digital Droplet PCR

AirID-Based Proximity Labeling for Protein-Protein Interaction in Plants

Visualizing Subcellular Localization of a Protein in the Heart Using Quantum Dots-Mediated Immuno-Labeling Followed by Transmission Electron Microscopy

A Standardized Liquid Biopsy Preanalytical Protocol for Downstream Circulating-Free DNA Applications

An R-Based Landscape Validation of a Competing Risk Model

Freeze-Fracture Electron Microscopy for Extracellular Vesicle Analysis

High Spatial Resolution Chemical Imaging of Implant-Associated Infections with X-ray Excited Luminescence Chemical Imaging Through Tissue

Constructing Cyclic Peptides Using an On-Tether Sulfonium Center

Construction of Cyclic Cell-Penetrating Peptides for Enhanced Penetration of Biological Barriers

Intramyocardial Injection for the Study of Cardiac Lymphatic Function in Zebrafish

Investigating Long-Distance Transport of Perfluoroalkyl Acids in Wheat via a Split-Root Exposure Technique

Source and Route of Pyrrolizidine Alkaloid Contamination in Tea Samples

C. elegans Gonad Dissection and Freeze Crack for Immunofluorescence and DAPI Staining

A Mouse Model for the Transition of Streptococcus pneumoniae from Colonizer to Pathogen upon Viral Co-Infection Recapitulates Age-Exacerbated Illness

Phage-Mediated Genetic Manipulation of the Lyme Disease Spirochete Borrelia burgdorferi

Preparation, Characteristics, Toxicity, and Efficacy Evaluation of the Nasal Self-Assembled Nanoemulsion Tumor Vaccine In Vitro and In Vivo

A Modified Murine Heterotopic Heart Transplant Protocol Matching Contemporary Standards of Aseptic Technique, Anesthesia, and Analgesia

Isolation and Quantification of Epstein-Barr Virus from the P3HR1 Cell Line

Contact Hypersensitivity as a Murine Model of Allergic Contact Dermatitis

Detection of Polyfunctional T Cells in Children Vaccinated with Japanese Encephalitis Vaccine via the Flow Cytometry Technique

A Soluble Tetrazolium-Based Reduction Assay to Evaluate the Effect of Antibodies on Candida tropicalis Biofilms

Zebrafish Animal Model for the Study of Allergic Reactions in Response to Tick Saliva Biomolecules

A Novel High-Throughput Ex Vivo Ovine Skin Wound Model for Testing Emerging Antibiotics

Ex Vivo Analysis of Mechanically Activated Ca2+ Transients in Urothelial Cells

Image Acquisition using Portable Sonography for Emergency Airway Management

Infrared Thermography for the Detection of Changes in Brown Adipose Tissue Activity

Bridging the Technology Divide in the COVID-19 Era: Using Virtual Outreach to Expose Middle and High School Students to Imaging Technology

Modified Posterior Vertebral Column Resection for Patients with Thoracolumbar Kyphotic Deformity

C-arm-Free Simultaneous OLIF51 and Percutaneous Pedicle Screw Fixation in a Single Lateral Position

Electromagnetic Controlled Closed-Head Model of Mild Traumatic Brain Injury in Mice

Imaging the Aging Cochlea with Light-Sheet Fluorescence Microscopy

Modeling Human Cerebellar Development In Vitro in 2D Structure

Evaluation and Manipulation of Neural Activity Using Two-Photon Holographic Microscopy

Fabrication and Use of Dry Macroporous Alginate Scaffolds for Viral Transduction of T Cells

Microengineering 3D Collagen Hydrogels with Long-Range Fiber Alignment

In Vitro Selection of Aptamers to Differentiate Infectious from Non-Infectious Viruses

Cellular Affinity of Particle-Stabilized Emulsion to Boost Antigen Internalization

Compost Microcosms as Microbially Diverse, Natural-like Environments for Microbiome Research in Caenorhabditis elegans

Super-Resolution Microscopy of the Synaptonemal Complex Within the Caenorhabditis elegans Germline

CRISPR-Cas9-Mediated Precise Knock-In Edits in Zebrafish Hearts

Isolation of Conditionally Immortalized Mouse Glomerular Endothelial Cells with Fluorescent Mitochondria

Embryo Injection Technique for Gene Editing in the Black-Legged Tick, Ixodes scapularis

Hand Dissection of Caenorhabditis elegans Intestines

Genetic Screen for Identification of Multicopy Suppressors in Schizosaccharomyces pombe

Embryo Rescue Protocol for Interspecific Hybridization in Squash

Induction and Diverse Assessment Indicators of Experimental Autoimmune Encephalomyelitis

A Flow Cytometry-Based Cell Surface Protein Binding Assay for Assessing Selectivity and Specificity of an Anticancer Aptamer

Far-Red Fluorescent Senescence-Associated β-Galactosidase Probe for Identification and Enrichment of Senescent Tumor Cells by Flow Cytometry

Digital Spatial Profiling for Characterization of the Microenvironment in Adult-Type Diffusely Infiltrating Glioma

Evaluation of the Spindle Assembly Checkpoint Integrity in Mouse Oocytes

An Efficient Clearing Protocol for the Study of Seed Development in Tomato (Solanum lycopersicum L.)

Microtensiometer for Confocal Microscopy Visualization of Dynamic Interfaces

Triplet Fusion Upconversion Nanocapsule Synthesis

An Effective Inoculation Method for Phytophthora capsici on Black Pepper Plants

Transient Transduction of the Strobilated Forms of Echinococcus granulosus

Detection of Homologous Recombination Intermediates via Proximity Ligation and Quantitative PCR in Saccharomyces cerevisiae

A Mouse Model of Orotracheal Intubation and Ventilated Lung Ischemia Reperfusion Surgery

Separation of Immune Cell Subpopulations in Peripheral Blood Samples from Children with Infectious Mononucleosis

Dynamic Continuous Blood Extraction from Rat Heart via Noninvasive Microdialysis Technique

Transdermal Measurement of Glomerular Filtration Rate in Mechanically Ventilated Piglets

Laparoscopic Non-Mesh Cerclage Pectopexy for Pelvic Organ Prolapse

Laser-Induced Action Potential-Like Measurements of Cardiomyocytes on Microelectrode Arrays for Increased Predictivity of Safety Pharmacology

Dynamic Navigation for Dental Implant Placement

A Two-Step Method for Percutaneous Transhepatic Choledochoscopic Lithotomy

Indocyanine Green-Guided Intraoperative Imaging to Facilitate Video-Assisted Retroperitoneal Debridement for Treating Acute Necrotizing Pancreatitis

In Vivo Luminal Measurement of Distension-Evoked Urothelial ATP Release in Rodents

The Transition to an Anterior-Based Muscle Sparing Approach Improves Early Postoperative Function but is Associated with a Learning Curve

Subcellular Fractionation for the Isolation of Synaptic Components from the Murine Brain

Through a Dog's Eyes: fMRI Decoding of Naturalistic Videos from the Dog Cortex

Ex Vivo Analysis of Mechanically Activated Ca²⁺ Transients in Urothelial Cells