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Genetic Screen for Identification of Multicopy Suppressors in Schizosaccharomyces pombe

1.8K Views

13:22 min

September 13th, 2022

DOI :

10.3791/63967-v

September 13th, 2022


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Genetic Screen

Hoofdstukken in deze video

0:04

Introduction

1:00

Transforming cDNA Library into the QuerySchizosaccharomyces pombe Mutant Strain and Screening Multicopy Suppressors

5:16

Validating the Suppressors of the Phenotype Associated with the Query Mutant Strain

7:41

Isolation of the Plasmid from the Schizosaccharomyces pombeSuppressor Clones

9:55

Identification of the Gene Encoded by the Suppressor Clone

10:48

Results: Screening Multicopy Suppressor of ell1 Deletion-Associated Genotoxic Stress Sensitivity

12:49

Conclusion

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