JoVE Table of Contents: Issue 163, September 2020

Multimodal Protocol for Assessing Metacognition and Self-Regulation in Adults with Learning Difficulties

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Journal (Behavior)

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Methods Collections

A Chronic Sleep Fragmentation Model using Vibrating Orbital Rotor to Induce Cognitive Deficit and Anxiety-Like Behavior in Young Wild-Type Mice

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Journal (Behavior)

Working Memory Training for Older Participants: A Control Group Training Regimen and Initial Intellectual Functioning Assessment

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Journal (Behavior)

PCR Mutagenesis, Cloning, Expression, Fast Protein Purification Protocols and Crystallization of the Wild Type and Mutant Forms of Tryptophan Synthase

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Journal (Biochemistry)

Ultra-High-Speed Western Blot using Immunoreaction Enhancing Technology

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Journal (Biochemistry)

Cell-Free Production of Proteoliposomes for Functional Analysis and Antibody Development Targeting Membrane Proteins

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Journal (Biochemistry)

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Methods Collections

Single Cell Analysis Of Transcriptionally Active Alleles By Single Molecule FISH

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Journal (Biochemistry)

Rapid and Cost-Effective RNA Extraction of Rat Pancreatic Tissue

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Journal (Biochemistry)

An <em>In Vitro</em> Single-Molecule Imaging Assay for the Analysis of Cap-Dependent Translation Kinetics

An In Vitro Single-Molecule Imaging Assay for the Analysis of Cap-Dependent Translation Kinetics

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Journal (Biochemistry)

A Model Membrane Platform for Reconstituting Mitochondrial Membrane Dynamics

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Journal (Biochemistry)

Enabling Real-Time Compensation in Fast Photochemical Oxidations of Proteins for the Determination of Protein Topography Changes

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Journal (Biochemistry)

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Methods Collections

Generating a Fractal Microstructure of Laminin-111 to Signal to Cells

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Journal (Bioengineering)

Transplantation of a 3D Bioprinted Patch in a Murine Model of Myocardial Infarction

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Journal (Bioengineering)

Lipidico Injection Protocol for Serial Crystallography Measurements at the Australian Synchrotron

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Journal (Bioengineering)

Asymmetrical Flow Field-Flow Fractionation for Sizing of Gold Nanoparticles in Suspension

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Journal (Bioengineering)

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Methods Collections

Imaging and Quantification of the Area of Fast-Moving Microbubbles Using a High-Speed Camera and Image Analysis

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Journal (Bioengineering)

Detergent-Free Decellularization of the Human Pancreas for Soluble Extracellular Matrix (ECM) Production

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Journal (Bioengineering)

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Methods Collections

Image Rendering Techniques in Postmortem Computed Tomography: Evaluation of Biological Health and Profile in Stranded Cetaceans

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Journal (Biology)

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Methods Collections

Collection of Skeletal Muscle Biopsies from the Superior Compartment of Human Musculus Tibialis Anterior for Mechanical Evaluation

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Journal (Biology)

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Methods Collections

Quantification of Circulating Pig-Specific DNA in the Blood of a Xenotransplantation Model

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Journal (Biology)

Graphene Enclosure of Chemically Fixed Mammalian Cells for Liquid-Phase Electron Microscopy

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Journal (Biology)

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Methods Collections

Quantifying Spatiotemporal Parameters of Cellular Exocytosis in Micropatterned Cells

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Journal (Biology)

A Bacterial Oral Feeding Assay with Antibiotic-Treated Mosquitoes

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Journal (Biology)

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Methods Collections

Assessment of de novo Protein Synthesis Rates in <em>Caenorhabditis elegans</em>

Assessment of de novo Protein Synthesis Rates in Caenorhabditis elegans

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Journal (Biology)

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Methods Collections

Demonstration of Heterologous Complexes formed by Golgi-Resident Type III Membrane Proteins using Split Luciferase Complementation Assay

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Journal (Biology)

Preparing and Injecting Embryos of <em>Culex</em> Mosquitoes to Generate Null Mutations using CRISPR/Cas9

Preparing and Injecting Embryos of Culex Mosquitoes to Generate Null Mutations using CRISPR/Cas9

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Journal (Biology)

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Methods Collections

Exosome Isolation after in vitro Shock Wave Therapy

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Journal (Biology)

Simultaneous Live Imaging of Multiple Insect Embryos in Sample Chamber-Based Light Sheet Fluorescence Microscopes

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Journal (Biology)

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Methods Collections

Immunohistochemistry Techniques to Analyze Cellular Proliferation and Neurogenesis in Rats Using the Thymidine Analog BrdU

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Journal (Biology)

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Methods Collections

Photogrammetric Three-dimensional Modeling and Printing of Cetacean Skeleton using an Omura's Whale Stranded in Hong Kong Waters as an Example

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Journal (Biology)

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Methods Collections

Cryogenic Sample Loading into a Magic Angle Spinning Nuclear Magnetic Resonance Spectrometer that Preserves Cellular Viability

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Journal (Biology)

An Orthotopic Resectional Mouse Model of Pancreatic Cancer

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Journal (Cancer Research)

In Vitro Evaluation of Oncogenic Transformation in Human Mammary Epithelial Cells

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Journal (Cancer Research)

Transradial Access Chemoembolization for Hepatocellular Carcinoma Patients

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Journal (Cancer Research)

Genome-Wide Analysis of DNA Methylation in Gastrointestinal Cancer

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Journal (Cancer Research)

Detection of Cell-Free DNA in Blood Plasma Samples of Cancer Patients

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Journal (Cancer Research)

Modeling Primary Bone Tumors and Bone Metastasis with Solid Tumor Graft Implantation into Bone

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Journal (Cancer Research)

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Methods Collections

Zinc-Sponge Battery Electrodes that Suppress Dendrites

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Journal (Chemistry)

Versatile Dual-Inlet Sample Introduction System for Multi-Mode Single Particle Inductively Coupled Plasma Mass Spectrometry Analysis and Validation

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Journal (Chemistry)

Localized Bathless Metal-Composite Plating via Electrostamping

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Journal (Chemistry)

Atomic Force Microscopy Combined with Infrared Spectroscopy as a Tool to Probe Single Bacterium Chemistry

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Journal (Chemistry)

Preparation of Nanoparticles for ToF-SIMS and XPS Analysis

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Journal (Chemistry)

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Methods Collections

Chromatographic Fingerprinting by Template Matching for Data Collected by Comprehensive Two-Dimensional Gas Chromatography

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Journal (Chemistry)

Real-Time Imaging of CCL5-Induced Migration of Periosteal Skeletal Stem Cells in Mice

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Journal (Developmental Biology)

Whole Animal Imaging of <em>Drosophila melanogaster</em> using Microcomputed Tomography

Whole Animal Imaging of Drosophila melanogaster using Microcomputed Tomography

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Journal (Developmental Biology)

Demonstration of Spin-Multiplexed and Direction-Multiplexed All-Dielectric Visible Metaholograms

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Journal (Engineering)

Application of a Coupling Agent to Improve the Dielectric Properties of Polymer-Based Nanocomposites

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Journal (Engineering)

Assessing Intertidal Populations of the Invasive European Green Crab

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Journal (Environment)

Experimental Protocol for Detecting Mitochondrial Function in Hepatocytes Exposed to Organochlorine Pesticides

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Journal (Environment)

Two-Dimensional Visualization and Quantification of Labile, Inorganic Plant Nutrients and Contaminants in Soil

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Journal (Environment)

A Suppressor Screen for the Characterization of Genetic Links Regulating Chronological Lifespan in <em>Saccharomyces cerevisiae</em>

A Suppressor Screen for the Characterization of Genetic Links Regulating Chronological Lifespan in Saccharomyces cerevisiae

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Journal (Genetics)

Use of Fimbrial Rod for F18ab Fimbriae<sup>+</sup> STEC Colonization to Host Cells

Use of Fimbrial Rod for F18ab Fimbriae⁺ STEC Colonization to Host Cells

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Journal (Immunology and Infection)

Distinguishing Intrapulmonary Immune Cells from Intravascular Immune Cell Populations: the Intrajugular Approach

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Journal (Immunology and Infection)

Polarization of M1 and M2 Human Monocyte-Derived Cells and Analysis with Flow Cytometry upon <em>Mycobacterium tuberculosis</em> Infection

Polarization of M1 and M2 Human Monocyte-Derived Cells and Analysis with Flow Cytometry upon Mycobacterium tuberculosis Infection

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Journal (Immunology and Infection)

Constructing Mutants in Serotype 1 <em>Streptococcus pneumoniae</em> strain 519/43

Constructing Mutants in Serotype 1 Streptococcus pneumoniae strain 519/43

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Journal (Immunology and Infection)

Assessment of Dependence in Activities of Daily Living Among Older Patients in an Acute Care Unit

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Journal (Medicine)

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Methods Collections

Evaluation of Capnography Sampling Line Compatibility and Accuracy when Used with a Portable Capnography Monitor

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Journal (Medicine)

Halogenated Agent Delivery in Porcine Model of Acute Respiratory Distress Syndrome via an Intensive Care Unit Type Device

Predicting Amputation using Local Circulating Mononuclear Progenitor Cells in Angioplasty-treated Patients with Critical Limb Ischemia

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Journal (Medicine)

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Methods Collections

Intramyocardial Transplantation of MSC-Loading Injectable Hydrogels after Myocardial Infarction in a Murine Model

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Journal (Medicine)

Oxygen-Induced Retinopathy Model for Ischemic Retinal Diseases in Rodents

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Journal (Medicine)

The Lublin Protocol of the Uterine Arteries Embolization in the Treatment of Symptomatic Uterine Fibroids

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Journal (Medicine)

Transaxillary First Rib Resection for Treatment of the Thoracic Outlet Syndrome

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Journal (Medicine)

A Quantitative Detection Method for MicroRNAs in the Kidney of an Ischemic Kidney Injury Mouse Model

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Journal (Medicine)

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Methods Collections

Operating Transverse Aortic Constriction with Absorbable Suture to Obtain Transient Myocardial Hypertrophy

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Journal (Medicine)

Electroretinogram Recording for Infants and Children under Anesthesia to Achieve Optimal Dark Adaptation and International Standards

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Journal (Medicine)

Delayed Intramyocardial Delivery of Stem Cells after Ischemia Reperfusion Injury in a Murine Model

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Journal (Medicine)

Culturing Rat Sympathetic Neurons from Embryonic Superior Cervical Ganglia for Morphological and Proteomic Analysis

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Journal (Neuroscience)

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Methods Collections

Laser-Induced Brain Injury in the Motor Cortex of Rats

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Journal (Neuroscience)

Three-Dimensional Motor Nerve Organoid Generation

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Journal (Neuroscience)

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Methods Collections

Real-Time fMRI Brain Mapping in Animals

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Journal (Neuroscience)

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Methods Collections

Horizontal Hippocampal Slices of the Mouse Brain

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Journal (Neuroscience)

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Methods Collections

Evaluating Postural Control and Lower-extremity Muscle Activation in Individuals with Chronic Ankle Instability

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Journal (Neuroscience)

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Methods Collections

Quantitative Approaches for Scoring <em>in vivo</em> Neuronal Aggregate and Organelle Extrusion in Large Exopher Vesicles in <em>C. elegans</em>

Quantitative Approaches for Scoring in vivo Neuronal Aggregate and Organelle Extrusion in Large Exopher Vesicles in C. elegans

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Journal (Neuroscience)

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Methods Collections

Implantation of Osmotic Pumps and Induction of Stress to Establish a Symptomatic, Pharmacological Mouse Model for DYT/PARK-ATP1A3 Dystonia

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Journal (Neuroscience)

Preparing Acute Brain Slices from the Dorsal Pole of the Hippocampus from Adult Rodents

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Journal (Neuroscience)

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Methods Collections

Quantifying Spontaneous Ca<sup>2+</sup> Fluxes and their Downstream Effects in Primary Mouse Midbrain Neurons

Quantifying Spontaneous Ca²⁺ Fluxes and their Downstream Effects in Primary Mouse Midbrain Neurons

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Journal (Neuroscience)

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Methods Collections

Combined In Vivo Anatomical and Functional Tracing of Ventral Tegmental Area Glutamate Terminals in the Hippocampus

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Journal (Neuroscience)

Sheath-Preserving Optic Nerve Transection in Rats to Assess Axon Regeneration and Interventions Targeting the Retinal Ganglion Cell Axon

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Journal (Neuroscience)

Unraveling the Role of Discrete Areas of the Rat Brain in the Regulation of Ovulation through Reversible Inactivation by Tetrodotoxin Microinjections

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Journal (Neuroscience)

Intracerebroventricular Treatment with Resiniferatoxin and Pain Tests in Mice

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Journal (Neuroscience)

Inhoudsopgave

Archiveren: Issue 163, September 2020

GA NAAR

Multimodal Protocol for Assessing Metacognition and Self-Regulation in Adults with Learning Difficulties

A Chronic Sleep Fragmentation Model using Vibrating Orbital Rotor to Induce Cognitive Deficit and Anxiety-Like Behavior in Young Wild-Type Mice

Working Memory Training for Older Participants: A Control Group Training Regimen and Initial Intellectual Functioning Assessment

PCR Mutagenesis, Cloning, Expression, Fast Protein Purification Protocols and Crystallization of the Wild Type and Mutant Forms of Tryptophan Synthase

Ultra-High-Speed Western Blot using Immunoreaction Enhancing Technology

Cell-Free Production of Proteoliposomes for Functional Analysis and Antibody Development Targeting Membrane Proteins

Single Cell Analysis Of Transcriptionally Active Alleles By Single Molecule FISH

Rapid and Cost-Effective RNA Extraction of Rat Pancreatic Tissue

An In Vitro Single-Molecule Imaging Assay for the Analysis of Cap-Dependent Translation Kinetics

A Model Membrane Platform for Reconstituting Mitochondrial Membrane Dynamics

Enabling Real-Time Compensation in Fast Photochemical Oxidations of Proteins for the Determination of Protein Topography Changes

Generating a Fractal Microstructure of Laminin-111 to Signal to Cells

Transplantation of a 3D Bioprinted Patch in a Murine Model of Myocardial Infarction

Lipidico Injection Protocol for Serial Crystallography Measurements at the Australian Synchrotron

Asymmetrical Flow Field-Flow Fractionation for Sizing of Gold Nanoparticles in Suspension

Imaging and Quantification of the Area of Fast-Moving Microbubbles Using a High-Speed Camera and Image Analysis

Detergent-Free Decellularization of the Human Pancreas for Soluble Extracellular Matrix (ECM) Production

Image Rendering Techniques in Postmortem Computed Tomography: Evaluation of Biological Health and Profile in Stranded Cetaceans

Collection of Skeletal Muscle Biopsies from the Superior Compartment of Human Musculus Tibialis Anterior for Mechanical Evaluation

Quantification of Circulating Pig-Specific DNA in the Blood of a Xenotransplantation Model

Graphene Enclosure of Chemically Fixed Mammalian Cells for Liquid-Phase Electron Microscopy

Quantifying Spatiotemporal Parameters of Cellular Exocytosis in Micropatterned Cells

A Bacterial Oral Feeding Assay with Antibiotic-Treated Mosquitoes

Assessment of de novo Protein Synthesis Rates in Caenorhabditis elegans

Demonstration of Heterologous Complexes formed by Golgi-Resident Type III Membrane Proteins using Split Luciferase Complementation Assay

Preparing and Injecting Embryos of Culex Mosquitoes to Generate Null Mutations using CRISPR/Cas9

Exosome Isolation after in vitro Shock Wave Therapy

Simultaneous Live Imaging of Multiple Insect Embryos in Sample Chamber-Based Light Sheet Fluorescence Microscopes

Immunohistochemistry Techniques to Analyze Cellular Proliferation and Neurogenesis in Rats Using the Thymidine Analog BrdU

Photogrammetric Three-dimensional Modeling and Printing of Cetacean Skeleton using an Omura's Whale Stranded in Hong Kong Waters as an Example

Cryogenic Sample Loading into a Magic Angle Spinning Nuclear Magnetic Resonance Spectrometer that Preserves Cellular Viability

An Orthotopic Resectional Mouse Model of Pancreatic Cancer

In Vitro Evaluation of Oncogenic Transformation in Human Mammary Epithelial Cells

Transradial Access Chemoembolization for Hepatocellular Carcinoma Patients

Genome-Wide Analysis of DNA Methylation in Gastrointestinal Cancer

Detection of Cell-Free DNA in Blood Plasma Samples of Cancer Patients

Modeling Primary Bone Tumors and Bone Metastasis with Solid Tumor Graft Implantation into Bone

Zinc-Sponge Battery Electrodes that Suppress Dendrites

Versatile Dual-Inlet Sample Introduction System for Multi-Mode Single Particle Inductively Coupled Plasma Mass Spectrometry Analysis and Validation

Localized Bathless Metal-Composite Plating via Electrostamping

Atomic Force Microscopy Combined with Infrared Spectroscopy as a Tool to Probe Single Bacterium Chemistry

Preparation of Nanoparticles for ToF-SIMS and XPS Analysis

Chromatographic Fingerprinting by Template Matching for Data Collected by Comprehensive Two-Dimensional Gas Chromatography

Real-Time Imaging of CCL5-Induced Migration of Periosteal Skeletal Stem Cells in Mice

Whole Animal Imaging of Drosophila melanogaster using Microcomputed Tomography

Demonstration of Spin-Multiplexed and Direction-Multiplexed All-Dielectric Visible Metaholograms

Application of a Coupling Agent to Improve the Dielectric Properties of Polymer-Based Nanocomposites

Assessing Intertidal Populations of the Invasive European Green Crab

Experimental Protocol for Detecting Mitochondrial Function in Hepatocytes Exposed to Organochlorine Pesticides

Two-Dimensional Visualization and Quantification of Labile, Inorganic Plant Nutrients and Contaminants in Soil

A Suppressor Screen for the Characterization of Genetic Links Regulating Chronological Lifespan in Saccharomyces cerevisiae

Use of Fimbrial Rod for F18ab Fimbriae+ STEC Colonization to Host Cells

Distinguishing Intrapulmonary Immune Cells from Intravascular Immune Cell Populations: the Intrajugular Approach

Polarization of M1 and M2 Human Monocyte-Derived Cells and Analysis with Flow Cytometry upon Mycobacterium tuberculosis Infection

Constructing Mutants in Serotype 1 Streptococcus pneumoniae strain 519/43

Assessment of Dependence in Activities of Daily Living Among Older Patients in an Acute Care Unit

Evaluation of Capnography Sampling Line Compatibility and Accuracy when Used with a Portable Capnography Monitor

Halogenated Agent Delivery in Porcine Model of Acute Respiratory Distress Syndrome via an Intensive Care Unit Type Device

Predicting Amputation using Local Circulating Mononuclear Progenitor Cells in Angioplasty-treated Patients with Critical Limb Ischemia

Intramyocardial Transplantation of MSC-Loading Injectable Hydrogels after Myocardial Infarction in a Murine Model

Oxygen-Induced Retinopathy Model for Ischemic Retinal Diseases in Rodents

The Lublin Protocol of the Uterine Arteries Embolization in the Treatment of Symptomatic Uterine Fibroids

Transaxillary First Rib Resection for Treatment of the Thoracic Outlet Syndrome

A Quantitative Detection Method for MicroRNAs in the Kidney of an Ischemic Kidney Injury Mouse Model

Operating Transverse Aortic Constriction with Absorbable Suture to Obtain Transient Myocardial Hypertrophy

Electroretinogram Recording for Infants and Children under Anesthesia to Achieve Optimal Dark Adaptation and International Standards

Delayed Intramyocardial Delivery of Stem Cells after Ischemia Reperfusion Injury in a Murine Model

Culturing Rat Sympathetic Neurons from Embryonic Superior Cervical Ganglia for Morphological and Proteomic Analysis

Targeted Neuronal Injury for the Non-Invasive Disconnection of Brain Circuitry

Laser-Induced Brain Injury in the Motor Cortex of Rats

Three-Dimensional Motor Nerve Organoid Generation

Real-Time fMRI Brain Mapping in Animals

Horizontal Hippocampal Slices of the Mouse Brain

Evaluating Postural Control and Lower-extremity Muscle Activation in Individuals with Chronic Ankle Instability

Quantitative Approaches for Scoring in vivo Neuronal Aggregate and Organelle Extrusion in Large Exopher Vesicles in C. elegans

Implantation of Osmotic Pumps and Induction of Stress to Establish a Symptomatic, Pharmacological Mouse Model for DYT/PARK-ATP1A3 Dystonia

Preparing Acute Brain Slices from the Dorsal Pole of the Hippocampus from Adult Rodents

Use of Fimbrial Rod for F18ab Fimbriae⁺ STEC Colonization to Host Cells

Quantifying Spontaneous Ca²⁺ Fluxes and their Downstream Effects in Primary Mouse Midbrain Neurons