JoVE Table of Contents: Issue 169, March 2021

Iterative Development of an Innovative Smartphone-Based Dietary Assessment Tool: Traqq

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Journal (Behavior)

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Methods Collections

Transplantation of Neonatal Mouse Cardiac Macrophages into Adult Mice

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Journal (Biochemistry)

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Methods Collections

Crystallization and Structural Determination of an Enzyme:Substrate Complex by Serial Crystallography in a Versatile Microfluidic Chip

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Journal (Biochemistry)

Identifying the Binding Proteins of Small Ligands with the Differential Radial Capillary Action of Ligand Assay (DRaCALA)

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Journal (Biochemistry)

$Microcrystal Electron Diffraction of Small Molecules$

Microcrystal Electron Diffraction of Small Molecules

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Journal (Biochemistry)

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Methods Collections

Fluorescence-Based Measurements of Phosphatidylserine/Phosphatidylinositol 4-Phosphate Exchange Between Membranes

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Journal (Biochemistry)

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Methods Collections

Ganglioside Extraction, Purification and Profiling

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Journal (Biochemistry)

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Methods Collections

Measuring Nucleotide Binding to Intact, Functional Membrane Proteins in Real Time

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Journal (Biochemistry)

Visualizing Diffusional Dynamics of Gold Nanorods on Cell Membrane using Single Nanoparticle Darkfield Microscopy

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Journal (Biochemistry)

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Methods Collections

Isolation of Histone from Sorghum Leaf Tissue for Top Down Mass Spectrometry Profiling of Potential Epigenetic Markers

Analysis and Specification of Starch Granule Size Distributions

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Journal (Biochemistry)

Size Exclusion Chromatography to Analyze Bacterial Outer Membrane Vesicle Heterogeneity

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Journal (Bioengineering)

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Methods Collections

Microinjection of Corn Planthopper, <em>Peregrinus maidis</em>, Embryos for CRISPR/Cas9 Genome Editing

Microinjection of Corn Planthopper, Peregrinus maidis, Embryos for CRISPR/Cas9 Genome Editing

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Journal (Bioengineering)

Production of Membrane-Filtered Phase-Shift Decafluorobutane Nanodroplets from Preformed Microbubbles

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Journal (Bioengineering)

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Methods Collections

DNA Tension Probes to Map the Transient Piconewton Receptor Forces by Immune Cells

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Journal (Bioengineering)

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Methods Collections

Functional Transcranial Doppler Ultrasound for Monitoring Cerebral Blood Flow

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Journal (Bioengineering)

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Methods Collections

<em>In Vitro</em> 3D Cell-Cultured Arterial Models for Studying Vascular Drug Targeting Under Flow

In Vitro 3D Cell-Cultured Arterial Models for Studying Vascular Drug Targeting Under Flow

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Journal (Bioengineering)

Fabrication of 3D Cardiac Microtissue Arrays using Human iPSC-Derived Cardiomyocytes, Cardiac Fibroblasts, and Endothelial Cells

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Journal (Bioengineering)

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Methods Collections

Measuring 3D In-vivo Shoulder Kinematics using Biplanar Videoradiography

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Journal (Bioengineering)

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Methods Collections

Toxicity Screens in Human Retinal Organoids for Pharmaceutical Discovery

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Journal (Bioengineering)

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Methods Collections

Two-Step Reverse Transcription Droplet Digital PCR Protocols for SARS-CoV-2 Detection and Quantification

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Journal (Biology)

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Methods Collections

Delivery of the Cas9/sgRNA Ribonucleoprotein Complex in Immortalized and Primary Cells via Virus-like Particles ("Nanoblades")

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Journal (Biology)

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Methods Collections

Systematic Approach to Identify Novel Antimicrobial and Antibiofilm Molecules from Plants' Extracts and Fractions to Prevent Dental Caries

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Journal (Biology)

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Methods Collections

Dissection of the Endolymphatic Sac from Mice

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Journal (Biology)

Nanoparticle Tracking Analysis for the Quantification and Size Determination of Extracellular Vesicles

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Journal (Biology)

Staining and High-Resolution Imaging of Three-Dimensional Organoid and Spheroid Models

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Journal (Biology)

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Methods Collections

Serial Block-Face Scanning Electron Microscopy (SBF-SEM) of Biological Tissue Samples

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Journal (Biology)

Culture Methods to Study Apical-Specific Interactions using Intestinal Organoid Models

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Journal (Biology)

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Methods Collections

Chromatin Extraction from Frozen Chimeric Liver Tissue for Chromatin Immunoprecipitation Analysis

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Journal (Biology)

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Methods Collections

Strategies for Optimization of Cryogenic Electron Tomography Data Acquisition

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Journal (Biology)

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Methods Collections

Live-cell Imaging of Single-Cell Arrays (LISCA) - a Versatile Technique to Quantify Cellular Kinetics

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Journal (Biology)

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Methods Collections

A 3D Cartographic Description of the Cell by Cryo Soft X-ray Tomography

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Journal (Biology)

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Methods Collections

Optimization of Crystal Growth for Neutron Macromolecular Crystallography

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Journal (Biology)

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Methods Collections

Preparing Irradiated and Marked Male <em>Aedes aegypti</em> Mosquitoes for Release in an Operational Sterile Insect Technique Program

Preparing Irradiated and Marked Male Aedes aegypti Mosquitoes for Release in an Operational Sterile Insect Technique Program

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Journal (Biology)

Irradiator Commissioning and Dosimetry for Assessment of LQ α and β Parameters, Radiation Dosing Schema, and in vivo Dose Deposition

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Journal (Biology)

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Methods Collections

A Step-by-Step Guide to Mosquito Electroantennography

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Journal (Biology)

An Easy and Flexible Inoculation Method for Accurately Assessing Powdery Mildew-Infection Phenotypes of Arabidopsis and Other Plants

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Journal (Biology)

Particle Templated Emulsification enables Microfluidic-Free Droplet Assays

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Journal (Biology)

Field-Based Thermal Physiology Assay: Cold Shock Recovery under Ambient Conditions

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Journal (Biology)

In vivo Measurement of Knee Extensor Muscle Function in Mice

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Journal (Biology)

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Methods Collections

Fluorescence In Situ Hybridization on DNA Halo Preparations to Reveal Whole Chromosomes, Telomeres and Gene Loci

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Journal (Biology)

Assessing Agrochemical Risk to Mated Honey Bee Queens

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Journal (Biology)

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Methods Collections

A Rapid Screening Workflow to Identify Potential Combination Therapy for GBM using Patient-Derived Glioma Stem Cells

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Journal (Cancer Research)

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Methods Collections

An Orthotopic Mouse Model of Ovarian Cancer using Human Stroma to Promote Metastasis

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Journal (Cancer Research)

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Methods Collections

Zebrafish Model of Neuroblastoma Metastasis

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Journal (Cancer Research)

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Methods Collections

Functional Analysis of Tumor-Infiltrating Myeloid Cells by Flow Cytometry and Adoptive Transfer

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Journal (Cancer Research)

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Methods Collections

Phase Change Dimethyldioctadecylammonium-Shelled Microdroplets as a Promising Drug Delivery System: Results on 3D Spheroids of Mammalian Tumor Cells

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Journal (Chemistry)

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Methods Collections

Monitoring Protein-Ligand Interactions in Human Cells by Real-Time Quantitative In-Cell NMR using a High Cell Density Bioreactor

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Journal (Chemistry)

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Methods Collections

Ultrafast Lignin Extraction from Unusual Mediterranean Lignocellulosic Residues

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Journal (Chemistry)

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Methods Collections

Microscopic Visualization of Porous Nanographenes Synthesized through a Combination of Solution and On-Surface Chemistry

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Journal (Chemistry)

High-Resolution Studies of Proteins in Natural Membranes by Solid-State NMR

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Journal (Chemistry)

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Methods Collections

In situ Grazing Incidence Small Angle X-ray Scattering on Roll-To-Roll Coating of Organic Solar Cells with Laboratory X-ray Instrumentation

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Journal (Chemistry)

Directed Differentiation of Hemogenic Endothelial Cells from Human Pluripotent Stem Cells

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Journal (Developmental Biology)

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Methods Collections

Using Chicken Embryo as a Powerful Tool in Assessment of Developmental Cardiotoxicities

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Journal (Developmental Biology)

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Methods Collections

A Microfluidics Approach for the Functional Investigation of Signaling Oscillations Governing Somitogenesis

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Journal (Developmental Biology)

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Methods Collections

Is My Mouse Pregnant? High-Frequency Ultrasound Assessment

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Journal (Developmental Biology)

Solution Blow Spinning of Polymeric Nano-Composite Fibers for Personal Protective Equipment

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Journal (Engineering)

Deep Neural Networks for Image-Based Dietary Assessment

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Journal (Engineering)

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Methods Collections

Aesthetically Enhanced Silica Aerogel Via Incorporation of Laser Etching and Dyes

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Journal (Engineering)

Building an Enhanced Flight Mill for the Study of Tethered Insect Flight

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Journal (Engineering)

Visualizing Lung Cellular Adaptations during Combined Ozone and LPS Induced Murine Acute Lung Injury

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Journal (Immunology and Infection)

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Methods Collections

Production of Human CRISPR-Engineered CAR-T Cells

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Journal (Immunology and Infection)

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Methods Collections

Isolating Brown Adipocytes from Murine Interscapular Brown Adipose Tissue for Gene and Protein Expression Analysis

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Journal (Immunology and Infection)

Stability and Structure of Bat Major Histocompatibility Complex Class I with Heterologous β<sub>2</sub>-Microglobulin

Stability and Structure of Bat Major Histocompatibility Complex Class I with Heterologous β₂-Microglobulin

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Journal (Immunology and Infection)

Focal Laser Ablation of Prostate Cancer: An Office Procedure

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Journal (Medicine)

Multi-system Monitoring for Identification of Seizures, Arrhythmias and Apnea in Conscious Restrained Rabbits

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Journal (Medicine)

Fertility Preservation in Patients with Severe Ovarian Dysfunction

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Journal (Medicine)

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Methods Collections

Preparation of Peripheral Blood Mononuclear Cell Pellets and Plasma from a Single Blood Draw at Clinical Trial Sites for Biomarker Analysis

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Journal (Medicine)

Preparation of Decellularized Kidney Scaffolds in Rats

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Journal (Medicine)

Digital Handwriting Analysis of Characters in Chinese Patients with Mild Cognitive Impairment

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Journal (Medicine)

Virtual Reality Tools for Assessing Unilateral Spatial Neglect: A Novel Opportunity for Data Collection

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Journal (Medicine)

Robotic Radical Cystectomy, Pelvic Lymph Node Dissection, and Intracorporeal Ileal Conduit Urinary Diversion

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Journal (Medicine)

Organ Ischemia-Reperfusion Injury by Simulating Hemodynamic Changes in Rat Liver Transplant Model

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Journal (Medicine)

Real-Time Magnetic Resonance Guided Focused Ultrasound for Painful Bone Metastases

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Journal (Medicine)

Short-Duration Hypothermia Induction in Rats using Models for Studies examining Clinical Relevance and Mechanisms

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Journal (Medicine)

Sarcomere Shortening of Pluripotent Stem Cell-Derived Cardiomyocytes using Fluorescent-Tagged Sarcomere Proteins.

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Journal (Medicine)

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Methods Collections

Simplifying Transcutaneous Intratracheal Drug Delivery in the Newborn Preterm Rabbit

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Journal (Medicine)

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Methods Collections

Cone-Enriched Cultures from the Retina of Chicken Embryos to Study Rod to Cone Cellular Interactions

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Journal (Neuroscience)

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Methods Collections

Time-Lapse Imaging of Neuronal Arborization using Sparse Adeno-Associated Virus Labeling of Genetically Targeted Retinal Cell Populations

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Journal (Neuroscience)

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Methods Collections

Reliable Acquisition of Electroencephalography Data during Simultaneous Electroencephalography and Functional MRI

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Journal (Neuroscience)

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Methods Collections

A Model of Epileptogenesis in Rhinal Cortex-Hippocampus Organotypic Slice Cultures

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Journal (Neuroscience)

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Methods Collections

Multiplexed Analysis of Retinal Gene Expression and Chromatin Accessibility Using scRNA-Seq and scATAC-Seq

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Journal (Neuroscience)

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Methods Collections

Motor Dual-Tasks for Gait Analysis and Evaluation in Post-Stroke Patients

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Journal (Neuroscience)

Microsurgical Dissection and Tissue Clearing for High Resolution Intact Whole Retina and Vitreous Imaging

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Journal (Neuroscience)

High-Content Screening Differentiation and Maturation Analysis of Fetal and Adult Neural Stem Cell-Derived Oligodendrocyte Precursor Cell Cultures

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Journal (Neuroscience)

Immunostaining of Whole-Mount Retinas with the CLARITY Tissue Clearing Method

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Journal (Neuroscience)

Inhoudsopgave

Archiveren: Issue 169, March 2021

GA NAAR

Iterative Development of an Innovative Smartphone-Based Dietary Assessment Tool: Traqq

Transplantation of Neonatal Mouse Cardiac Macrophages into Adult Mice

Crystallization and Structural Determination of an Enzyme:Substrate Complex by Serial Crystallography in a Versatile Microfluidic Chip

Identifying the Binding Proteins of Small Ligands with the Differential Radial Capillary Action of Ligand Assay (DRaCALA)

Microcrystal Electron Diffraction of Small Molecules

Fluorescence-Based Measurements of Phosphatidylserine/Phosphatidylinositol 4-Phosphate Exchange Between Membranes

Ganglioside Extraction, Purification and Profiling

Measuring Nucleotide Binding to Intact, Functional Membrane Proteins in Real Time

Visualizing Diffusional Dynamics of Gold Nanorods on Cell Membrane using Single Nanoparticle Darkfield Microscopy

Isolation of Histone from Sorghum Leaf Tissue for Top Down Mass Spectrometry Profiling of Potential Epigenetic Markers

Analysis and Specification of Starch Granule Size Distributions

Workflow and Tools for Crystallographic Fragment Screening at the Helmholtz-Zentrum Berlin

Size Exclusion Chromatography to Analyze Bacterial Outer Membrane Vesicle Heterogeneity

Microinjection of Corn Planthopper, Peregrinus maidis, Embryos for CRISPR/Cas9 Genome Editing

Production of Membrane-Filtered Phase-Shift Decafluorobutane Nanodroplets from Preformed Microbubbles

DNA Tension Probes to Map the Transient Piconewton Receptor Forces by Immune Cells

Functional Transcranial Doppler Ultrasound for Monitoring Cerebral Blood Flow

In Vitro 3D Cell-Cultured Arterial Models for Studying Vascular Drug Targeting Under Flow

Fabrication of 3D Cardiac Microtissue Arrays using Human iPSC-Derived Cardiomyocytes, Cardiac Fibroblasts, and Endothelial Cells

Measuring 3D In-vivo Shoulder Kinematics using Biplanar Videoradiography

Toxicity Screens in Human Retinal Organoids for Pharmaceutical Discovery

Two-Step Reverse Transcription Droplet Digital PCR Protocols for SARS-CoV-2 Detection and Quantification

Delivery of the Cas9/sgRNA Ribonucleoprotein Complex in Immortalized and Primary Cells via Virus-like Particles ("Nanoblades")

Systematic Approach to Identify Novel Antimicrobial and Antibiofilm Molecules from Plants' Extracts and Fractions to Prevent Dental Caries

Dissection of the Endolymphatic Sac from Mice

Nanoparticle Tracking Analysis for the Quantification and Size Determination of Extracellular Vesicles

Staining and High-Resolution Imaging of Three-Dimensional Organoid and Spheroid Models

Serial Block-Face Scanning Electron Microscopy (SBF-SEM) of Biological Tissue Samples

Culture Methods to Study Apical-Specific Interactions using Intestinal Organoid Models

Chromatin Extraction from Frozen Chimeric Liver Tissue for Chromatin Immunoprecipitation Analysis

Strategies for Optimization of Cryogenic Electron Tomography Data Acquisition

Live-cell Imaging of Single-Cell Arrays (LISCA) - a Versatile Technique to Quantify Cellular Kinetics

A 3D Cartographic Description of the Cell by Cryo Soft X-ray Tomography

Optimization of Crystal Growth for Neutron Macromolecular Crystallography

Preparing Irradiated and Marked Male Aedes aegypti Mosquitoes for Release in an Operational Sterile Insect Technique Program

Irradiator Commissioning and Dosimetry for Assessment of LQ α and β Parameters, Radiation Dosing Schema, and in vivo Dose Deposition

A Step-by-Step Guide to Mosquito Electroantennography

An Easy and Flexible Inoculation Method for Accurately Assessing Powdery Mildew-Infection Phenotypes of Arabidopsis and Other Plants

Particle Templated Emulsification enables Microfluidic-Free Droplet Assays

Field-Based Thermal Physiology Assay: Cold Shock Recovery under Ambient Conditions

In vivo Measurement of Knee Extensor Muscle Function in Mice

Fluorescence In Situ Hybridization on DNA Halo Preparations to Reveal Whole Chromosomes, Telomeres and Gene Loci

Assessing Agrochemical Risk to Mated Honey Bee Queens

Automated Dissection Protocol for Tumor Enrichment in Low Tumor Content Tissues

A Rapid Screening Workflow to Identify Potential Combination Therapy for GBM using Patient-Derived Glioma Stem Cells

An Orthotopic Mouse Model of Ovarian Cancer using Human Stroma to Promote Metastasis

Zebrafish Model of Neuroblastoma Metastasis

Functional Analysis of Tumor-Infiltrating Myeloid Cells by Flow Cytometry and Adoptive Transfer

Phase Change Dimethyldioctadecylammonium-Shelled Microdroplets as a Promising Drug Delivery System: Results on 3D Spheroids of Mammalian Tumor Cells

Monitoring Protein-Ligand Interactions in Human Cells by Real-Time Quantitative In-Cell NMR using a High Cell Density Bioreactor

Ultrafast Lignin Extraction from Unusual Mediterranean Lignocellulosic Residues

Microscopic Visualization of Porous Nanographenes Synthesized through a Combination of Solution and On-Surface Chemistry

High-Resolution Studies of Proteins in Natural Membranes by Solid-State NMR

In situ Grazing Incidence Small Angle X-ray Scattering on Roll-To-Roll Coating of Organic Solar Cells with Laboratory X-ray Instrumentation

Directed Differentiation of Hemogenic Endothelial Cells from Human Pluripotent Stem Cells

Using Chicken Embryo as a Powerful Tool in Assessment of Developmental Cardiotoxicities

A Microfluidics Approach for the Functional Investigation of Signaling Oscillations Governing Somitogenesis

Is My Mouse Pregnant? High-Frequency Ultrasound Assessment

Solution Blow Spinning of Polymeric Nano-Composite Fibers for Personal Protective Equipment

Deep Neural Networks for Image-Based Dietary Assessment

Aesthetically Enhanced Silica Aerogel Via Incorporation of Laser Etching and Dyes

Building an Enhanced Flight Mill for the Study of Tethered Insect Flight

Visualizing Lung Cellular Adaptations during Combined Ozone and LPS Induced Murine Acute Lung Injury

Production of Human CRISPR-Engineered CAR-T Cells

Establishing Transcription Profile of Psoriasiform Cutaneous In Vitro using HaCaT Cells Stimulated with Combination of Cytokines

Isolating Brown Adipocytes from Murine Interscapular Brown Adipose Tissue for Gene and Protein Expression Analysis

Stability and Structure of Bat Major Histocompatibility Complex Class I with Heterologous β2-Microglobulin

Focal Laser Ablation of Prostate Cancer: An Office Procedure

Multi-system Monitoring for Identification of Seizures, Arrhythmias and Apnea in Conscious Restrained Rabbits

Fertility Preservation in Patients with Severe Ovarian Dysfunction

Segmentation and Linear Measurement for Body Composition Analysis using Slice-O-Matic and Horos

Preparation of Peripheral Blood Mononuclear Cell Pellets and Plasma from a Single Blood Draw at Clinical Trial Sites for Biomarker Analysis

Preparation of Decellularized Kidney Scaffolds in Rats

Digital Handwriting Analysis of Characters in Chinese Patients with Mild Cognitive Impairment

Virtual Reality Tools for Assessing Unilateral Spatial Neglect: A Novel Opportunity for Data Collection

Robotic Radical Cystectomy, Pelvic Lymph Node Dissection, and Intracorporeal Ileal Conduit Urinary Diversion

Organ Ischemia-Reperfusion Injury by Simulating Hemodynamic Changes in Rat Liver Transplant Model

Stability and Structure of Bat Major Histocompatibility Complex Class I with Heterologous β₂-Microglobulin