JoVE Journal

Immunology and Infection

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Nanyang Technological University

התפתחות Lyophilized Loop בתיווך ריאגנטים הגברה איזו תרמים לצורך זיהוי של

We described here a simple loop-mediated isothermal amplification (LAMP) method using lyophilized reagents for the detection of C. burnetii in patient samples.

Chapters in this video

0:05

Title

0:40

Prepare Plasmid DNA Dilutions as Standard for LAMP Reaction

1:24

Perform Standard LAMP Reaction

2:54

Perform LAMP Reaction with Reconstituted Reagents

5:17

Results: Detection of Coxiella burnetii with the LAMP Reaction

6:37

Conclusion

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