JoVE Table of Contents: Issue 175, September 2021

Handling Techniques to Reduce Stress in Mice

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Journal (Behavior)

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Methods Collections

An Affordable and Efficient "Homemade" Platform for <em>Drosophila</em> Behavioral Studies, and an Accompanying Protocol for Larval Mitochondrial Respirometry

An Affordable and Efficient "Homemade" Platform for Drosophila Behavioral Studies, and an Accompanying Protocol for Larval Mitochondrial Respirometry

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Journal (Behavior)

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Methods Collections

Animal Models of Depression - Chronic Despair Model (CDM)

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Journal (Behavior)

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Methods Collections

Assessment of Spatial Lingual Tactile Sensitivity using a Gratings Orientation Test

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Journal (Behavior)

Problem-Solving Before Instruction (PS-I): A Protocol for Assessment and Intervention in Students with Different Abilities

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Journal (Behavior)

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Methods Collections

<em>N-</em>glycan Profiling of Glycoproteins by Hydrophilic Interaction Liquid Chromatography with Fluorescence and Mass Spectrometric Detection

N-glycan Profiling of Glycoproteins by Hydrophilic Interaction Liquid Chromatography with Fluorescence and Mass Spectrometric Detection

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Journal (Biochemistry)

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Methods Collections

Paramagnetic Relaxation Enhancement for Detecting and Characterizing Self-Associations of Intrinsically Disordered Proteins

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Journal (Biochemistry)

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Methods Collections

Generating Self-Assembling Human Heart Organoids Derived from Pluripotent Stem Cells

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Journal (Biochemistry)

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Methods Collections

A Reporter Based Cellular Assay for Monitoring Splicing Efficiency

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Journal (Biochemistry)

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Methods Collections

Visualization and Quantification of TGFβ/BMP/SMAD Signaling under Different Fluid Shear Stress Conditions using Proximity-Ligation-Assay

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Journal (Biochemistry)

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Methods Collections

Micropatterning Transmission Electron Microscopy Grids to Direct Cell Positioning within Whole-Cell Cryo-Electron Tomography Workflows

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Journal (Biochemistry)

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Methods Collections

Time-resolved Förster Resonance Energy Transfer Assays for Measurement of Endogenous Phosphorylated STAT Proteins in Human Cells

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Journal (Biochemistry)

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Methods Collections

Measurement of Fatty Acid β-Oxidation in a Suspension of Freshly Isolated Mouse Hepatocytes

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Journal (Biochemistry)

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Methods Collections

Single-Molecule Imaging of EWS-FLI1 Condensates Assembling on DNA

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Journal (Biochemistry)

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Methods Collections

Spatio-Temporal <em>In Vivo</em> Imaging of Ocular Drug Delivery Systems using Fiberoptic Confocal Laser Microendoscopy

Spatio-Temporal In Vivo Imaging of Ocular Drug Delivery Systems using Fiberoptic Confocal Laser Microendoscopy

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Journal (Bioengineering)

<em>In vitro</em> Induction of Human Dental Pulp Stem Cells Toward Pancreatic Lineages

In vitro Induction of Human Dental Pulp Stem Cells Toward Pancreatic Lineages

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Journal (Bioengineering)

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Methods Collections

$Liquid Chromatography Coupled to Refractive Index or Mass Spectrometric Detection for Metabolite Profiling in Lysate-based Cell-free Systems$

Liquid Chromatography Coupled to Refractive Index or Mass Spectrometric Detection for Metabolite Profiling in Lysate-based Cell-free Systems

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Journal (Bioengineering)

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Methods Collections

Establishing an Octopus Ecosystem for Biomedical and Bioengineering Research

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Journal (Bioengineering)

<em>In Vitro</em> Multiparametric Cellular Analysis by Micro Organic Charge-modulated Field-effect Transistor Arrays

In Vitro Multiparametric Cellular Analysis by Micro Organic Charge-modulated Field-effect Transistor Arrays

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Journal (Bioengineering)

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Methods Collections

High-Dimensionality Flow Cytometry for Immune Function Analysis of Dissected Implant Tissues

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Journal (Bioengineering)

A High-Yield <em>Streptomyces</em> Transcription-Translation Toolkit for Synthetic Biology and Natural Product Applications

A High-Yield Streptomyces Transcription-Translation Toolkit for Synthetic Biology and Natural Product Applications

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Journal (Bioengineering)

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Methods Collections

Decellularization for the Preparation of Highly Preserved Human Acellular Skin Matrix for Regenerative Medicine

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Journal (Bioengineering)

Preparation and Characterization of Targeted Microbubbles

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Journal (Bioengineering)

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Methods Collections

Detection of <em>Plasmodium</em> Sporozoites in <em>Anopheles</em> Mosquitoes using an Enzyme-linked Immunosorbent Assay

Detection of Plasmodium Sporozoites in Anopheles Mosquitoes using an Enzyme-linked Immunosorbent Assay

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Journal (Biology)

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Methods Collections

Dissection and Immunostaining of Larval Salivary Glands from <em>Anopheles gambiae</em> Mosquitoes

Dissection and Immunostaining of Larval Salivary Glands from Anopheles gambiae Mosquitoes

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Journal (Biology)

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Methods Collections

Tail Vein Transection Bleeding Model in Fully Anesthetized Hemophilia A Mice

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Journal (Biology)

Isolation and Selection of Entomopathogenic Fungi from Soil Samples and Evaluation of Fungal Virulence against Insect Pests

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Journal (Biology)

Imaging Molecular Adhesion in Cell Rolling by Adhesion Footprint Assay

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Journal (Biology)

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Methods Collections

Divergence of Root Microbiota in Different Habitats based on Weighted Correlation Networks

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Journal (Biology)

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Methods Collections

Assessment of Cellular Bioenergetics in Mouse Hematopoietic Stem and Primitive Progenitor Cells using the Extracellular Flux Analyzer

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Journal (Biology)

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Methods Collections

Isolation and Culture of Primary Oral Keratinocytes from the Adult Mouse Palate

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Journal (Biology)

Hyperactive <em>piggyBac</em> Transposase-mediated Germline Transformation in the Fall Armyworm, <em>Spodoptera frugiperda</em>

Hyperactive piggyBac Transposase-mediated Germline Transformation in the Fall Armyworm, Spodoptera frugiperda

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Journal (Biology)

Nuclei Isolation from Adult Mouse Kidney for Single-Nucleus RNA-Sequencing

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Journal (Biology)

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Methods Collections

Live-Cell Imaging of Transcriptional Activity at DNA Double-Strand Breaks

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Journal (Biology)

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Methods Collections

Intracavernous Pressure Recording in a Cavernous Nerve Injury Rat Model

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Journal (Biology)

Analyzing Oxidative Stress in Murine Intestinal Organoids using Reactive Oxygen Species-Sensitive Fluorogenic Probe

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Journal (Biology)

Isolation of Human Neutrophils from Whole Blood and Buffy Coats

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Journal (Biology)

Detecting Virus and Salivary Proteins of a Leafhopper Vector in the Plant Host

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Journal (Biology)

Automated Detection and Analysis of Exocytosis

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Journal (Biology)

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Methods Collections

Planarian Ovary Dissection for Ultrastructural Analysis and Antibody Staining

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Journal (Biology)

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Methods Collections

Improved Enzyme Protection Assay to Study <em>Staphylococcus aureus</em> Internalization and Intracellular Efficacy of Antimicrobial Compounds

Improved Enzyme Protection Assay to Study Staphylococcus aureus Internalization and Intracellular Efficacy of Antimicrobial Compounds

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Journal (Biology)

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Methods Collections

<em>In Situ</em> Exploration of Murine Megakaryopoiesis using Transmission Electron Microscopy

In Situ Exploration of Murine Megakaryopoiesis using Transmission Electron Microscopy

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Journal (Biology)

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Methods Collections

Quantifying Tissue-Specific Proteostatic Decline in <em>Caenorhabditis elegans</em>

Quantifying Tissue-Specific Proteostatic Decline in Caenorhabditis elegans

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Journal (Biology)

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Methods Collections

<em>In Vivo</em> Measurement of Hindlimb Dorsiflexor Isometric Torque from Pig

In Vivo Measurement of Hindlimb Dorsiflexor Isometric Torque from Pig

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Journal (Biology)

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Methods Collections

Quantification of Interbacterial Competition using Single-Cell Fluorescence Imaging

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Journal (Biology)

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Methods Collections

A Three-Dimensional Spheroid Model to Investigate the Tumor-Stromal Interaction in Hepatocellular Carcinoma

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Journal (Cancer Research)

<em>In Vivo</em> Immunogenicity Screening of Tumor-Derived Extracellular Vesicles by Flow Cytometry of Splenic T Cells

In Vivo Immunogenicity Screening of Tumor-Derived Extracellular Vesicles by Flow Cytometry of Splenic T Cells

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Journal (Cancer Research)

Expansion and Enrichment of Gamma-Delta (γδ) T Cells from Apheresed Human Product

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Journal (Cancer Research)

An <em>Ex Vivo</em> Brain Slice Model to Study and Target Breast Cancer Brain Metastatic Tumor Growth

An Ex Vivo Brain Slice Model to Study and Target Breast Cancer Brain Metastatic Tumor Growth

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Journal (Cancer Research)

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Methods Collections

Three Differential Expression Analysis Methods for RNA Sequencing: limma, EdgeR, DESeq2

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Journal (Cancer Research)

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Methods Collections

Analysis of Lymph Node Volume by Ultra-High-Frequency Ultrasound Imaging in the Braf/Pten Genetically Engineered Mouse Model of Melanoma

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Journal (Cancer Research)

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Methods Collections

Aqueous Humor as a Liquid Biopsy for Retinoblastoma: Clear Corneal Paracentesis and Genomic Analysis

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Journal (Cancer Research)

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Methods Collections

Ortho- and Ectopic Zebrafish Xeno-Engraftment of Ocular Melanoma to Recapitulate Primary Tumor and Experimental Metastasis Development

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Journal (Cancer Research)

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Methods Collections

Portal Vein Injection of Colorectal Cancer Organoids to Study the Liver Metastasis Stroma

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Journal (Cancer Research)

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Methods Collections

Radiosynthesis of 1-(2-[<sup>18</sup>F]Fluoroethyl)-L-Tryptophan using a One-pot, Two-step Protocol

Radiosynthesis of 1-(2-[¹⁸F]Fluoroethyl)-L-Tryptophan using a One-pot, Two-step Protocol

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Journal (Chemistry)

Analyzing Melts and Fluids from Ab Initio Molecular Dynamics Simulations with the UMD Package

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Journal (Chemistry)

Accelerating Rate Calorimetry and Complementary Techniques to Characterize Battery Safety Hazards

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Journal (Chemistry)

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Methods Collections

Flow Cytometry and Confocal Imaging Analysis of Low Wnt Expression in Axin2-mTurquoise2 Reporter Thymocytes

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Journal (Developmental Biology)

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Methods Collections

Simultaneous Calcium Imaging and Glucose Stimulation in Living Zebrafish to Investigate In Vivo β-Cell Function

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Journal (Developmental Biology)

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Methods Collections

<em>In Ovo</em> Feeding of Commercial Broiler Eggs: An Accurate and Reproducible Method to Affect Muscle Development and Growth

In Ovo Feeding of Commercial Broiler Eggs: An Accurate and Reproducible Method to Affect Muscle Development and Growth

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Journal (Developmental Biology)

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Methods Collections

Whole Ovary Immunofluorescence, Clearing, and Multiphoton Microscopy for Quantitative 3D Analysis of the Developing Ovarian Reserve in Mouse

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Journal (Developmental Biology)

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Methods Collections

A Virtual Machine Platform for Non-Computer Professionals for Using Deep Learning to Classify Biological Sequences of Metagenomic Data

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Journal (Genetics)

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Methods Collections

<em>CcCIPK14</em> Gene Function Analysis to Illuminate the Efficient Root Transgenic System

CcCIPK14 Gene Function Analysis to Illuminate the Efficient Root Transgenic System

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Journal (Genetics)

In-Nucleus Hi-C in Drosophila Cells

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Journal (Genetics)

Indel Detection following CRISPR/Cas9 Mutagenesis using High-resolution Melt Analysis in the Mosquito <em>Aedes aegypti</em>

Indel Detection following CRISPR/Cas9 Mutagenesis using High-resolution Melt Analysis in the Mosquito Aedes aegypti

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Journal (Genetics)

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Methods Collections

Chromosome Screening of Human Preimplantation Embryos by Using Spent Culture Medium: Sample Collection and Chromosomal Ploidy Analysis

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Journal (Genetics)

A New Toolkit for Evaluating Gene Functions using Conditional Cas9 Stabilization

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Journal (Genetics)