JoVE Journal

Developmental Biology

You have full access to this content through

Nanyang Technological University

Efficient and Scalable Directed Differentiation of Clinically Compatible Corneal Limbal Epithelial Stem Cells from Human Pluripotent Stem Cells

This protocol introduces a simple two-step method for differentiating corneal limbal epithelial stem cells from human pluripotent stem cells under xeno- and feeder cell-free culture conditions. The cell culture methods presented here enable cost-efficient, large-scale production of clinical quality cells applicable to corneal cell therapy use.

Chapters in this video

0:04

Title

0:41

Step I: Corneal Induction in Suspension Culture

3:07

Step II: Corneal Differentiation in Adherent Culture

4:33

Step III: Cryo-banking Human Pluripotent Stem Cell-derived Limbal Epithelial Stem Cells

6:08

Step IV: Thawing the Cryopreserved Human Pluripotent Stem Cell-derived Limbal Epithelial Stem Cells

7:25

Results: Differentiation of Human Pluripotent Stem Cells to Clinically Compatible Corneal Limbal Epithelial Stem Cells

9:31

Conclusion

Related Videos

We use cookies to enhance your experience on our website.

By continuing to use our website or clicking “Continue”, you are agreeing to accept our cookies.

Learn More