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Nanyang Technological University

Large-Scale Production of Cardiomyocytes from Human Pluripotent Stem Cells Using a Highly Reproducible Small Molecule-Based Differentiation Protocol

10.5K Views

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12:21 min

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July 25th, 2016

DOI :

10.3791/54276-v

July 25th, 2016

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Hananeh Fonoudi*¹^,²^,³^,⁸, Hassan Ansari*¹^,⁸, Saeed Abbasalizadeh¹, Gillian M Blue⁶^,⁷, Nasser Aghdami¹, David S Winlaw⁶^,⁷, Richard P Harvey²^,³^,⁴, Alexis Bosman*²^,³, Hossein Baharvand*¹^,⁵

¹Department of Stem Cells and Developmental Biology, Cell Science Research Center, Royan Institute for Stem Cell Biology and Technology, ACECR, ²Developmental and Stem Cell Biology Division, Victor Chang Cardiac Research Institute, ³St. Vincent´s Clinical School, Faculty of Medicine, University of New South Wales, ⁴School of Biotechnology and Biomolecular Sciences, University of New South Wales, ⁵Department of Developmental Biology, University of Science and Culture, ⁶Heart Centre for Children, The Children´s Hospital at Westmead, ⁷Sydney Medical School, University of Sydney, ⁸Department of Developmental Biology, University of Science and Culture, Tehran, Iran

Summary

Here, we present a robust, fast and scalable cardiomyocyte differentiation protocol for human pluripotent stem cells (hPSCs). Cardiomyocytes derived using this large-scale method can provide sufficient cell numbers for their effective use in human cardiovascular disease modeling, high-throughput drug screening, and potentially clinical applications.

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Keyword Extraction Cardiomyocytes

Human Pluripotent Stem Cells

Differentiation Protocol

High Efficiency

Reproducibility

Cardiac Disease Modeling

Human Induced Pluripotent Stem Cells

Human Embryonic Stem Cells

Single Cell Passaging

Chapters in this video

0:05

Title

1:03

Spinner Flask Internal Surface Silicon Coating

2:42

hPSC Spheroid Stirred Suspension Bioreactor Differentiation

6:42

hPSC Differentiation Using Cultures Not Adapted to Single Cell Passaging

9:46

Results: Representative Cardiomyocyte Differentiation From Various hPSCs Cultures

11:19

Conclusion

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