Efficient and Scalable Directed Differentiation of Clinically Compatible Corneal Limbal Epithelial Stem Cells from Human Pluripotent Stem Cells

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7.6K Views

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10:07 min

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October 24th, 2018

DOI :

10.3791/58279-v

October 24th, 2018

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Heidi Hongisto*¹, Meri Vattulainen*¹, Tanja Ilmarinen¹, Alexandra Mikhailova², Heli Skottman¹

¹BioMediTech Institute, Faculty of Medicine and Life Sciences, University of Tampere, ²Department of Ophthalmology, SILK, Faculty of Medicine and Life Sciences, University of Tampere

Podsumowanie

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Keywords Corneal Limbal Epithelial Stem Cells

Human Pluripotent Stem Cells

Feeder free

Embryoid Body Formation

Basal Induction Medium

SB 505124

Human Basic Fibroblast Growth Factor

Limbal Stem Cell Deficiency

Corneal Cell Replacement Therapy

Rozdziały w tym wideo

0:04

Title

0:41

Step I: Corneal Induction in Suspension Culture

3:07

Step II: Corneal Differentiation in Adherent Culture

4:33

Step III: Cryo-banking Human Pluripotent Stem Cell-derived Limbal Epithelial Stem Cells

6:08

Step IV: Thawing the Cryopreserved Human Pluripotent Stem Cell-derived Limbal Epithelial Stem Cells

7:25

Results: Differentiation of Human Pluripotent Stem Cells to Clinically Compatible Corneal Limbal Epithelial Stem Cells

9:31

Conclusion

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