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Cancer Research

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Scalable Biomanufacturing Workflow to Produce and Isolate Natural Killer Cell-Derived Extracellular Vesicle-Based Cancer Biotherapeutics

557 Views

•

08:50 min

•

August 16th, 2024

DOI :

10.3791/67227-v

August 16th, 2024

•
1Biologic and Radiopharmaceutical Drugs Directorate, 2Department of Biochemistry, Microbiology and Immunology Institute, University of Ottawa, 3Centre for Infection, Immunity and Inflammation Institute, University of Ottawa, 4Regenerative Medicine Program, Ottawa Hospital Research Institute

Natural killer cell-derived extracellular vesicles (NK-EVs) hold promising potential as cancer biotherapeutics. This methodology-based study presents a scalable closed-loop biomanufacturing workflow designed to continuously produce and isolate large quantities of high-purity NK-EVs. In-process control testing is performed throughout the biomanufacturing workflow, ensuring the EVs meet quality standards for product release.

Explore More Videos

Biomanufacturing Workflow
Natural Killer Cells
Extracellular Vesicles
Cancer Biotherapeutics
Solid Tumors
Size Exclusion Chromatography
Product Purity
Vesicle Isolation
Therapeutic Potential
Protein Liquid Chromatography
EV rich Conditioned Media
Endonuclease Treatment
Preclinical Models
Chromatography System

Chapters in this video

0:00

Introduction

1:14

Purification and Sterilization of Natural Killer Cell Derived-Extracellular Vesicles (NK-EVs) Using Fast Protein Liquid Chromatography and Ultrafiltration for Nanoparticles Tracking Analysis

3:57

Filter-Based Sterilization and Concentration of NK-EV Product

5:16

Characterization of Natural Killer Cell Derived-Extracellular Vesicles (NK-EVs) Using Nanoparticle Tracking Analysis

7:53

Representative Results

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