A Three-dimensional Thymic Culture System to Generate Murine Induced Pluripotent Stem Cell-derived Tumor Antigen-specific Thymic Emigrants

7.1K Views

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10:44 min

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August 9th, 2019

DOI :

10.3791/58672-v

August 9th, 2019

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Raul Vizcardo*¹^,², S.M. Rafiqul Islam*¹^,², Takuya Maeda¹^,², Naritaka Tamaoki¹^,², Meghan L. Good¹^,², Nicholas D. Klemen¹^,², Marta Bosch-Marce¹^,², Li Jia¹, Mikhael J. Kruhlak³, Nicholas P. Restifo¹^,²

¹Surgery Branch, National Cancer Institute, NIH, ²Center for Cell-Based Therapy, National Cancer Institute, NIH, ³Laboratory of Cancer Biology and Genetics, Center for Cancer Research, National Cancer Institute, NIH

Summary

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3D Thymic Organ Culture

IPSC derived Thymic Emigrants

Tumor Antigen specific T Cells

Naive T Cell like Phenotype

CD8 Alpha beta T Cells

Cell Culture Procedure

OP9 DLL1 Cells

Trypsinization

Centrifugation

Cell Suspension Preparation

Tumor Suppression

Proliferation Capacity

Experimental Methodology

Chapters in this video

0:04

Title

0:57

Preparation of OP9/DLL1 Cells for Co-culture with iPSC

2:08

In Vitro Differentiation of iPSC into Immature T Cells

5:00

3D Thymic Organ Culture to Generate iTE

8:00

Results: Generation of Murine Induced Pluripotent Stem Cell-derived Tumor Antigen-specific Thymic Emigrants

9:46

Conclusion

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