Production of Human Neurogenin 2-Inducible Neurons in a Three-Dimensional Suspension Bioreactor
1.4K Views
•
07:21 min
•
March 17th, 2023
DOI :
March 17th, 2023
•Jeanette Wihan1, Isabell Karnatz1, Isabelle Sébastien1, Ralf Kettenhofen1, Benjamin Schmid2, Christian Clausen2, Benjamin Fischer1, Rachel Steeg3, Heiko Zimmermann1,4,5,6, Julia C. Neubauer1,4
1Fraunhofer Project Center for Stem Cell Process Engineering, Fraunhofer Institute for Biomedical Engineering IBMT, 2Bioneer A/S, 3Fraunhofer UK Research Ltd, Technology and Innovation Centre, 4Fraunhofer Institute for Biomedical Engineering IBMT, 5Department of Molecular and Cellular Biotechnology, Saarland University, 6Facultad de Ciencias del Mar, Universidad Católica del Norte
Explore More Videos
Chapters in this video
0:04
Introduction
0:51
Precultivation of hiPSCs in the Benchtop Bioreactor System (Day ‐2)
2:53
Differentiation of hiPSCs into Early Neurons (Day 0)
3:29
Cryopreservation of Early Neurons (Day 2)
5:01
Results: Assessing the Generation of hiPSC‐Derived Neurons Using the Benchtop Bioreactor
6:40
Conclusion
Related Videos
10:18
Three-dimensional Quantification of Dendritic Spines from Pyramidal Neurons Derived from Human Induced Pluripotent Stem Cells
11.4K Views
11:01
Cultivation of Human Neural Progenitor Cells in a 3-dimensional Self-assembling Peptide Hydrogel
16.5K Views
08:48
Synaptic Microcircuit Modeling with 3D Cocultures of Astrocytes and Neurons from Human Pluripotent Stem Cells
12.2K Views
15:05
Functional Evaluation of Biological Neurotoxins in Networked Cultures of Stem Cell-derived Central Nervous System Neurons
9.3K Views
09:07
Simple Generation of a High Yield Culture of Induced Neurons from Human Adult Skin Fibroblasts
10.2K Views
07:27
Rapid and Efficient Generation of Neurons from Human Pluripotent Stem Cells in a Multititre Plate Format
43.5K Views
08:03
Three-Dimensional Bioprinting of Human iPSC-Derived Neuron-Astrocyte Cocultures for Screening Applications
4.2K Views
09:34
Automated Production of Human Induced Pluripotent Stem Cell-Derived Cortical and Dopaminergic Neurons with Integrated Live-Cell Monitoring
7.1K Views
01:14
Generating 3D Co-culture Spheres of Astrocytes and Neurons to Induce Synapse Formation
43 Views
01:33
Generating Neurons by Reprogramming Human Dermal Fibroblast Cells
43 Views
Copyright © 2025 MyJoVE Corporation. All rights reserved