Automated Production of Human Induced Pluripotent Stem Cell-Derived Cortical and Dopaminergic Neurons with Integrated Live-Cell Monitoring
7.1K Views
•
09:34 min
•
August 6th, 2020
DOI :
August 6th, 2020
•Ashutosh Dhingra*1, Joachim Täger*1, Elisangela Bressan*1, Salvador Rodriguez-Nieto1, Manmeet-Sakshi Bedi1, Stefanie Bröer1, Eldem Sadikoglou1, Noémia Fernandes2, Melissa Castillo-Lizardo1, Patrizia Rizzu2, Peter Heutink1,3
1Genome Biology of Neurodegenerative Diseases, German Center for Neurodegenerative Diseases (DZNE), 2Applied Genomics for Neurodegenerative Diseases, German Center for Neurodegenerative Diseases (DZNE), 3Department for Neurodegenerative Diseases, Hertie Institute for Clinical Brain Research, University of Tübingen
Explore More Videos
Chapters in this video
0:05
Introduction
0:43
New Culture Plate and Tip Loading and Decontamination
1:35
Culture Medium and/or Dissociation Reagent Filling
2:09
Automated Method Execution
2:51
Human Induced Pluripotent Stem Cell (hiPSC) Seeding
4:09
Automated Confluence Assessment
4:47
Medium Change
5:37
Subcultivation
6:42
Automated High Content, High Throughput Imaging
7:14
Results: Representative Automated System-Produced hiPSC-Derived Cortical and Dopaminergic Neuron Characterization
8:58
Conclusion
Related Videos
10:18
Three-dimensional Quantification of Dendritic Spines from Pyramidal Neurons Derived from Human Induced Pluripotent Stem Cells
11.4K Views
09:20
Rapid Neuronal Differentiation of Induced Pluripotent Stem Cells for Measuring Network Activity on Micro-electrode Arrays
26.8K Views
10:47
Efficient Derivation of Human Neuronal Progenitors and Neurons from Pluripotent Human Embryonic Stem Cells with Small Molecule Induction
15.3K Views
06:40
Directed Dopaminergic Neuron Differentiation from Human Pluripotent Stem Cells
16.5K Views
09:36
Human Neural Organoids for Studying Brain Cancer and Neurodegenerative Diseases
9.8K Views
07:27
Rapid and Efficient Generation of Neurons from Human Pluripotent Stem Cells in a Multititre Plate Format
43.5K Views
00:06
Post-differentiation Replating of Human Pluripotent Stem Cell-derived Neurons for High-content Screening of Neuritogenesis and Synapse Maturation
9.2K Views
11:38
A Live-cell Image-Based Machine Learning Strategy to Monitor Pluripotent Stem Cell Differentiation
436 Views
01:38
Differentiating Human Induced Pluripotent Stem Cells into Neurons on Micro-Electrode Arrays
92 Views
01:29
Generation of Dopaminergic Neuronal Organoids from Human Embryonic Stem Cells
94 Views
Copyright © 2025 MyJoVE Corporation. All rights reserved