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Nanyang Technological University

A Simple Protocol for Mapping the Plant Root System Architecture Traits

2.6K Views

•

11:09 min

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February 10th, 2023

DOI :

10.3791/64876-v

February 10th, 2023

•

Devesh Shukla¹^,², Prabodh Kumar Trivedi²^,³, Shivendra Sahi¹^,⁴

¹Department of Biology, Western Kentucky University, ²Plant Biotechnology Division, CSIR Central Institute of Medicinal and Aromatic Plants, ³Academy of Scientific and Innovative Research (AcSIR), ⁴Department of Biology, St. Joseph's University

Summary

We use simple laboratory tools to examine the root system architecture (RSA) of Arabidopsis and Medicago. The plantlets are grown hydroponically over mesh and spread using an art brush to reveal the RSA. Images are taken using scanning or a high-resolution camera, then analyzed with ImageJ to map traits.

Explore More Videos

Plant Root System

Root System Architecture

Non invasive Hydroponics

Arabidopsis Seeds

Surface Sterilization

Environmental Interaction

Hormonal Conditions

Nutrient Conditions

Polypropylene Mesh

Hydroponic Growth

Experimental Media

Chapters in this video

0:04

Introduction

0:52

Arabidopsis Seed Surface Sterilization

2:23

Setting a Hydroponic System for Seed Germination

3:39

Examination of Root System Architecture (RSA)

4:07

Recording Images for Root System Architecture (RSA)

7:45

Results: Mapping the Plant Root System Architecture Traits

10:00

Conclusion

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