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Nanyang Technological University

Mycorrhizal Maps as a Tool to Explore Colonization Patterns and Fungal Strategies in the Roots of Festuca rubra and Zea mays

The protocol here describes the methods for the assessment of the arbuscular mycorrhizal colonization patterns and strategy in roots for two species: Zea mays and Festuca rubra. The use of the MycoPatt method permits the calculation of parameters, the conversion of mycorrhizal structures into digital data, and the mapping of their real position in roots.

Chapters in this video

0:05

Introduction

0:48

Root Processing, Clearing, and Staining for Microscopy

2:10

Root Processing for Microscopy

2:56

Microscopic Analysis of the Root Samples

3:26

Post-Microscopy Image Assemblage and Scoring of Mycorrhizal Colonization

5:05

Raw Data Analysis and Result Extraction

6:45

Results: Assessment of the Arbuscular Mycorrhizal Colonization Patterns

7:53

Conclusion

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