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Nanyang Technological University

Comparison of Methods for Isolating Entomopathogenic Fungi from Soil Samples

4.3K Views

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07:16 min

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January 6th, 2022

DOI :

10.3791/63353-v

January 6th, 2022

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Thais A. Correa¹, Fernanda S. Santos², Mariana G. Camargo³, Simone Quinelato², Vânia R. E. P. Bittencourt¹^,³, Patricia S. Golo¹^,³

¹Programa de Pós-Graduação em Ciências Veterinárias, Instituto de Veterinária, Universidade Federal Rural do Rio de Janeiro, ²Laboratório de Taxonomia Bioquímica e Bioprospecção de Fungos/Coleção de Cultura de Fungos Filamentosos, Instituto Oswaldo Cruz, Fundação Oswaldo Cruz (FIOCRUZ), ³Departamento de Parasitologia Animal, Instituto de Veterinária, Universidade Federal Rural do Rio de Janeiro

Summary

Entomopathogenic fungal colonies are isolated from tropical soil samples using Tenebrio bait, Galleria bait, as well as selective artificial medium, i.e., potato dextrose agar enriched with yeast extract supplemented with chloramphenicol, thiabendazole, and cycloheximide (CTC medium).

Explore More Videos

Entomopathogenic Fungi

Isolation Methods

Biopesticide Development

Chloramphenicol

Macro Morphology

Micro Morphology

Rhizosphere Competence

Biosafety Cabinet

Incubation Process

Galleria Mellonella

Tenebrio Molitor

Chapters in this video

0:04

Introduction

1:07

Soil Sampling and Isolation Methods for Entomopathogenic Fungi (EPF)

4:29

Identification of Entomopathogenic Fungi (EPF): Metarhizium and Beauveria Species

5:21

Results: Micromorphological Characteristics and Occurrence of Entomopathogenic Fungi in Various Soil Samples

6:16

Conclusion

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