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Nanyang Technological University

Isolation and Selection of Entomopathogenic Fungi from Soil Samples and Evaluation of Fungal Virulence against Insect Pests

9.1K Views

•

09:42 min

•

September 28th, 2021

DOI :

10.3791/62882-v

September 28th, 2021

•

Yao-Chia Liu¹, Nian-Tong Ni¹, Ju-Chun Chang¹, Yi-Hsuan Li¹, Mi Rong Lee², Jae Su Kim²^,³, Yu-Shin Nai¹

¹Department of Entomology, National Chung Hsing University, ²Department of Agricultural biology, Jeonbuk National University, ³Department of Agricultural Convergence Technology, Jeonbuk National University

Summary

Here we present a protocol based on the mealworm (Tenebrio molitor)-bait system that was used for isolating and selecting entomopathogenic fungi (EPF) from soil samples. An effective conidia number (ECN) formula is used to select high stress tolerant EPF based on physiological characteristics for pest microbial control in the field.

Explore More Videos

Entomopathogenic Fungi

Fungal Isolation

Microbial Control Agents

Virulence Evaluation

Pathogenicity Screening

Thermotolerance

Conidia Production

Biological Control

Tenebrio Molitor

Larva Mortality

Mycosis Observation

Sabouraud Dextrose Agar

Primary Culture

Fungal Isolates

Chapters in this video

0:04

Introduction

1:04

Isolation and Selection of Potential Entomopathogenic Fungi (EPF)

2:27

1st Pathogenicity Screen and 2nd Virulence Test of Fungal Isolates

3:42

Molecular and Morphological Identification of Potential Entomopathogenic Fungi (EPF)

5:23

Investigation of Conidial Productivity and Thermotolerance

7:07

Effective Conidia Number (ECN) Ranking

7:31

Results: Thermotolerance and Conidial Production Data into the ECN Formula and PCA Analysis

8:40

Conclusion

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