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Nanyang Technological University

Scanning Electron Microscopy (SEM) Protocols for Problematic Plant, Oomycete, and Fungal Samples

Problems in the processing of biological samples for scanning electron microscopy observation include cell collapse, treatment of samples from wet microenvironments and cell destruction. Low-cost and relatively rapid protocols suited for preparing challenging samples such as floral meristems, oomycete cysts, and fungi (Agaricales) are compiled and detailed here.

Chapters in this video

0:05

Title

0:57

Collection and Fixation of Developing and Fully Formed Plant Structures

2:04

Dissection and Dehydration of Developing and Fully Formed Plant Structures

3:02

Mounting and Preparing Plant Tissues for SEM Observation of Developing and Fully Formed Plant Structures

4:20

Study of Cyst Behavior of Saprolegnia (Oomycetes) on Different Surfaces

6:04

Study of Herbarium Fungal Spores of Phellorinia herculanea under SEM

7:20

Results: Recovery of Plant Development, Differential Spine Growth of Saprolegnia parasitica, and Nurse Cells in Spores of Phellorinia herculanea f. stellata

9:01

Conclusion

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