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Genetics
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Nanyang Technological UniversityUsing the GAL4-UAS System for Functional Genetics in Anopheles gambiae
5.9K Views
•
06:29 min
•
April 15th, 2021
DOI :
April 15th, 2021
•Chapters in this video
0:05
Introduction
0:49
Collection of Pupae for Microscopic Characterization and Identification of Fluorescent Markers
1:45
Sexing Pupae
2:25
Setting Up GAL4-UAS (Upstream Activation Sequences) System Crosses and Inducing Egg Laying
3:20
Embryo Fixation and Bleaching
4:15
Results: Analysis of Anopheles gambiae Pupae Expressing Fluorescent Markers Driven by 3xP3 Promoter, Tissue-specific Expression Visualization, and Embryo Clearing
5:53
Conclusion
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