JoVE Journal
Genetics
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Nanyang Technological UniversityDetermination of the Optimal Chromosomal Location(s) for a DNA Element in Escherichia coli Using a Novel Transposon-mediated Approach
7.5K Views
•
11:12 min
•
September 11th, 2017
DOI :
September 11th, 2017
•Chapters in this video
0:05
Title
0:48
Collection of Transposon Library
2:49
Competition Experiment in LB
3:50
Southern Blot Analysis to Monitor Competition Over Time
4:59
Identification of the Fittest Clones
7:06
Flow Cytometry
8:53
Results: Transposon-mediated Insertion of DNA Element in E. coli
10:41
Conclusion
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ISSN 2578-6326
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