Crystal Structure of the N-terminal Domain of Ryanodine Receptor from Plutella xylostella
7.4K Views
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11:31 min
•
November 30th, 2018
DOI :
November 30th, 2018
•Bidhan Chandra Nayak*1, Jie Wang*1, Lianyun Lin*1,2,3,4, Weiyi He2,3,4, Minsheng You2,3,4, Zhiguang Yuchi1,2
1Tianjin Key Laboratory for Modern Drug Delivery & High-Efficiency, Collaborative Innovation Center of Chemical Science and Engineering, School of Pharmaceutical Science and Technology, Tianjin University, 2State Key Laboratory of Ecological Pest Control for Fujian/Taiwan Crops and Institute of Applied Ecology, Fujian Agriculture and Forestry University, 3Joint International Research Laboratory of Ecological Pest Control, Ministry of Education, Fuzhou, 4Fujian-Taiwan Joint Centre for Ecological Control of Crop Pests, Fujian Agriculture and Forestry University
Chapters in this video
0:04
Title
1:01
Ligation-independent Cloning (LIC)
2:38
E. coli Transformation and Protein Expression
4:58
Fusion Protein Purification, Tag-cleaving, and Target Protein Re-purification
6:12
Protein Crystallization, Crystal Mounting, and Structure Determination
9:56
Results: Representative N-terminal Domain (NTD) Crystal Structure Determination
10:53
Conclusion
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