Analysis of Tubular Membrane Networks in Cardiac Myocytes from Atria and Ventricles

20.5K Views

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10:30 min

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October 15th, 2014

DOI :

10.3791/51823-v

October 15th, 2014

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Eva Wagner*¹^,²^,³, Sören Brandenburg*¹^,², Tobias Kohl¹^,², Stephan E. Lehnart¹^,²^,³^,⁴

¹Heart Research Center Goettingen, ²Clinic of Cardiology & Pulmonology, University Medical Center Goettingen, ³German Center for Cardiovascular Research (DZHK) partner site Goettingen, ⁴BioMET, Center for Biomedical Engineering & Technology, University of Maryland School of Medicine

Summary

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Keywords Cardiac Myocytes

Transverse axial Tubule System TATS

Atrial Myocytes

Ventricular Myocytes

Sarcoendoplasmic Reticulum

Calcium Release Units

Membrane Networks

Fluorescence Imaging

Confocal Microscopy

Superresolution Imaging

Image Analysis

Quantitative Analysis

Physiological Adaptation

Disease Changes

Chapters in this video

0:05

Title

1:46

Isolation of Atrial and Ventricular Myocytes from the Mouse Heart

4:09

Imaging of Transverse-axial Tubule System (TATS) Membrane Structures in Living Myocytes

5:41

Analysis of the TATS Membrane Network and its Components

8:23

Results: Representative TATS Component Orientations for Ventricular and Atrial Myocytes

9:26

Conclusion

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