Harvesting and Cryo-cooling Crystals of Membrane Proteins Grown in Lipidic Mesophases for Structure Determination by Macromolecular Crystallography
24.8K Views
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18:45 min
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September 2nd, 2012
DOI :
September 2nd, 2012
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Chapters in this video
0:00
Title
0:29
Materials and Equipment
2:12
Identifying Wells With Crystals
3:13
Opening the Well
4:04
"Closeup": Opening Wells with Cubic Phase - Method I
6:38
"Closeup": Opening Wells with Cubic Phase - Method 2
8:27
Opening Well with Sponge Phase - Unsuccessfully
10:25
Opening Well with Sponge Phase - Successfully
14:20
Harvesting Crystals
15:16
Harvesting Crystals from the Cubic Phase
16:12
Harvesting Crystals from the Sponge Phase
16:40
Cryo-Cooling Crystals
17:25
Storage/Transport Dewars
17:42
Crystal Images
18:03
Concluding Remarks
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