JoVE Journal
Biology
A subscription to JoVE is required to view this content.
Isolation of CD4+ T-cells and Analysis of Circulating T-follicular Helper (cTfh) Cell Subsets from Peripheral Blood Using 6-color Flow Cytometry
12.1K Views
•
07:39 min
•
January 7th, 2019
DOI :
January 7th, 2019
•Chapters in this video
0:04
Title
0:43
CD4+ T Cell Peripheral Whole Blood Isolation and Flow Cytometric Analysis
4:45
Data Analysis
6:07
Results: Representative Circulating T-follicular Helper (cTfh) Cell Subset Analyses
6:53
Conclusion
Related Videos
09:08
Isolating Human Peripheral Blood Mononuclear Cells and CD4+ T cells from Sézary Syndrome Patients for Transcriptomic Profiling
5.3K Views
10:14
Cell-based Flow Cytometry Assay to Measure Cytotoxic Activity
17.9K Views
11:50
Retroviral Transduction of Helper T Cells as a Genetic Approach to Study Mechanisms Controlling their Differentiation and Function
11.0K Views
09:01
Analysis of Human T Cell Activity in an Allogeneic Co-Culture Setting of Pre-Treated Tumor Cells
232 Views
08:06
Determination of Regulatory T Cell Subsets in Murine Thymus, Pancreatic Draining Lymph Node and Spleen Using Flow Cytometry
11.3K Views
10:58
Discrimination of Seven Immune Cell Subsets by Two-fluorochrome Flow Cytometry
13.7K Views
06:37
Flotation-Based T Cell Isolation, Activation, and Expansion from Human Peripheral Blood Mononuclear Cell Samples Using Microbubbles
2.4K Views
10:57
Cell Cycle Analysis: Assessing CD4 and CD8 T Cell Proliferation After Stimulation Using CFSE Staining and Flow Cytometry
24.2K Views
01:43
An In Vitro Technique to Study T Cell Interaction with Supported Planar Lipid Bilayers
109 Views
02:26
Selective Isolation of CD4+ Vδ1+ γδ T Cells from Human Peripheral Blood Mononuclear Cells
146 Views
Copyright © 2025 MyJoVE Corporation. All rights reserved
We use cookies to enhance your experience on our website.
By continuing to use our website or clicking “Continue”, you are agreeing to accept our cookies.