JoVE Journal

Immunology and Infection

You have full access to this content through

Nanyang Technological University

Flotation-Based T Cell Isolation, Activation, and Expansion from Human Peripheral Blood Mononuclear Cell Samples Using Microbubbles

Chapters in this video

0:04

Introduction

0:29

Isolation of T Cells with Microbubbles Using Positive Selection

1:51

Co-Stimulation (Activation) of the Positively Selected T Cells

2:52

Expansion of the Co-Stimulated Cells in Cell Culture Medium

3:28

Expansion of the T Cells (With or Without Prior Transduction)

4:02

Harvesting the T Cells and Flow Cytometry

5:16

Results: Assessment of Viable and Successfully Transduced Cells

5:58

Conclusion

Related Videos

We use cookies to enhance your experience on our website.

By continuing to use our website or clicking “Continue”, you are agreeing to accept our cookies.

Learn More