Retroviral Transduction of Helper T Cells as a Genetic Approach to Study Mechanisms Controlling their Differentiation and Function

1:01

Transfection by Calcium Phosphate Precipitation and Virus Collection

3:02

Isolation of Leukocyte Cells

4:13

Isolation of CD4⁺ T Cells Using Magnetic Beads Kit

6:02

Isolation of Naïve CD4⁺ T Cells Using Cell Separation Columns

8:10

Retroviral Transduction

9:37

Results: Analysis of Retrovirus-transfected HEK 293 Cells and Retrovirus Transduced Helper T Cells

11:16

Conclusion