JoVE Journal
Chemistry
A subscription to JoVE is required to view this content.
Simultaneous Measurement of HDAC1 and HDAC6 Activity in HeLa Cells Using UHPLC-MS
8.5K Views
•
09:20 min
•
August 10th, 2017
DOI :
August 10th, 2017
•Chapters in this video
0:05
Title
1:20
Cell Culture
3:00
Cell Treatment with Cell Compounds and HDAC Substrates, Cell Lysis, and Sample Preparation for UHPLC-ESI-MS/MS
5:14
UHPLC/MS-MS Analysis and Data Analysis
6:14
Results: Deacetylation of Specific Substrates by Endogenous HDACs and Potency of Standard HDAC Inhibitors
8:18
Conclusion
Related Videos
Single-Step Enrichment of a TAP-Tagged Histone Deacetylase of the Filamentous Fungus Aspergillus nidulans for Enzymatic Activity Assay
6.8K Views
Assays for Validating Histone Acetyltransferase Inhibitors
6.5K Views
Histone Modification Screening using Liquid Chromatography, Trapped Ion Mobility Spectrometry, and Time-Of-Flight Mass Spectrometry
1.0K Views
An Acetyl-Click Chemistry Assay to Measure Histone Acetyltransferase 1 Acetylation
764 Views
Expression Analysis of Mammalian Linker-histone Subtypes
13.8K Views
Purification of H3 and H4 Histone Proteins and the Quantification of Acetylated Histone Marks in Cells and Brain Tissue
21.2K Views
The ChroP Approach Combines ChIP and Mass Spectrometry to Dissect Locus-specific Proteomic Landscapes of Chromatin
18.1K Views
Discovering Protein Interactions and Characterizing Protein Function Using HaloTag Technology
36.9K Views
Examination of Proteins Bound to Nascent DNA in Mammalian Cells Using BrdU-ChIP-Slot-Western Technique
9.1K Views
Complete Workflow for Analysis of Histone Post-translational Modifications Using Bottom-up Mass Spectrometry: From Histone Extraction to Data Analysis
29.0K Views
Copyright © 2025 MyJoVE Corporation. All rights reserved
We use cookies to enhance your experience on our website.
By continuing to use our website or clicking “Continue”, you are agreeing to accept our cookies.