Discovering Protein Interactions and Characterizing Protein Function Using HaloTag Technology
36.9K Views
•
11:16 min
•
July 12th, 2014
DOI :
July 12th, 2014
•Chapters in this video
0:05
Title
1:23
Protein Pulldowns: Preparation of Transiently Transfected Cells
2:50
Equilibration of the HaloLink Resin
4:01
Binding and Washing of Fusion Complexes
6:21
SDS Elution for Denaturing Gels, Western Blots, or Mass Spectrometry
7:07
Cellular Imaging of Fluorescently Labeled Fusion Proteins Using a Cytoplasmic and Nuclear Permeable Ligand
8:48
Results: Using HaloTag Technology to Determine Protein Interactions and Localization
10:47
Conclusion
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