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Developmental Biology

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Nanyang Technological University

Use of Immunolabeling to Analyze Stable, Dynamic, and Nascent Microtubules in the Zebrafish Embryo

8.2K Views

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12:38 min

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September 20th, 2017

DOI :

10.3791/55792-v

September 20th, 2017

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Rebecca J. McFarland*¹, Sharlene P. Brown*¹, Eudorah Vital¹, Jonathan M. Werner¹, Rachel M. Brewster¹

¹Department of Biological Sciences, University of Maryland, Baltimore County

Summary

Immunolabeling methods to analyze distinct populations of microtubules in the developing zebrafish brain are described here, which are broadly applicable to other tissues. The first protocol outlines an optimized method for immunolabeling stable and dynamic microtubules. The second protocol provides a method to image and quantify nascent microtubules specifically.

Explore More Videos

Zebrafish Embryo

Developmental Neurobiology

Stable Microtubules

Dynamic Microtubules

Nascent Microtubules

Primary Antibodies

Secondary Antibodies

Chapters in this video

0:05

Title

0:45

Fixation of Staged Embryos

1:52

Sectioning for Immunolabeling

4:04

Confocal Imaging

5:17

Quantifying Microtubules

7:10

De Novo MT Assembly Assay

9:05

Depolymerize Existing MTs

10:26

Results: Analysis of Stable, Dynamic, and Nascent Microtubules in the Zebrafish Embryo

12:00

Conclusion

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