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Cancer Research

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Nanyang Technological University

Isolation of Circulating Tumor Cells in an Orthotopic Mouse Model of Colorectal Cancer

9.9K Views

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06:19 min

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July 18th, 2017

DOI :

10.3791/55357-v

July 18th, 2017

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Susan Kochall¹, May-Linn Thepkaysone¹, Sebastián A. García¹, Alexander M. Betzler¹, Jürgen Weitz¹^,²^,³, Christoph Reissfelder¹, Sebastian Schölch¹^,²^,³

¹Department of Gastrointestinal, Thoracic and Vascular Surgery, Medizinische Fakultät Carl Gustav Carus, Technische Universität Dresden, ²German Cancer Consortium (DKTK), ³German Cancer Research Center (DKFZ)

Summary

We describe the establishment of orthotopic colorectal tumors via injection of tumor cells or organoids into the cecum of mice and the subsequent isolation of circulating tumor cells (CTCs) from this model.

Explore More Videos

Circulating Tumor Cells

Orthotopic Mouse Model

Colorectal Cancer

Microsurgical Technique

Cecal Wall Injection

Reproducible Metastatic Tumors

Immunocompromised Mouse

Intra cecal Injection

Micro Injection Pump

Binocular Surgical Microscope

Density Gradient Medium

Blood Sample Collection

Chapters in this video

0:05

Title

0:40

Orthotopic Mouse Model

3:05

Circulating Tumor Cell (CTC) Isolation from Whole Blood

4:59

Results: Representative Murine Colorectal Cancer Induction

5:33

Conclusion

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