Analyzing Synaptic Modulation of Drosophila melanogaster Photoreceptors after Exposure to Prolonged Light

6.5K Views

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11:36 min

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February 10th, 2017

DOI :

10.3791/55176-v

February 10th, 2017

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Atsushi Sugie¹^,²^,⁵, Christoph Möhl³, Satoko Hakeda-Suzuki⁴, Hideaki Matsui¹^,², Takashi Suzuki*⁴, Gaia Tavosanis*⁵

¹Department of Neuroscience of Disease, Center for Transdisciplinary Research, Niigata University, ²Brain Research Institute, Niigata University, ³Image and Data Analysis Facility, German Center for Neurodegenerative Diseases (DZNE), ⁴Graduate School of Life Science and Technology, Tokyo Institute of Technology (Titech), ⁵Dendrite Differentiation, German Center for Neurodegenerative Diseases (DZNE)

Summary

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Keywords Synaptic Modulation

Drosophila Melanogaster

Photoreceptors

Prolonged Light Exposure

R7 And R8 Photoreceptor Axons

Chapters in this video

0:05

Title

0:47

Treatment of Flies and Imaging Brains

2:54

Image Analysis: Generating the Spots, Surface Objects, Start-points, and End-points

8:04

Image Analysis: Calculating the Number of Spots, Distribution of Spot Frequency and Enrichment Level of Brp-GFP

9:48

Results: Brp-GFP Expression in R8 Synapses of the Medulla

11:02

Conclusion

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