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Nanyang Technological University

Characterization of Calcification Events Using Live Optical and Electron Microscopy Techniques in a Marine Tubeworm

7.3K Views

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15:39 min

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February 28th, 2017

DOI :

10.3791/55164-v

February 28th, 2017

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Vera B. S. Chan¹, Takashi Toyofuku², George Wetzel³, Laxmikant Saraf³, Vengatesen Thiyagarajan⁴, Andrew S. Mount¹

¹Department of Biological Sciences, Clemson University, ²Department of Marine Biodiversity Research (BioDive), Japan Agency for Marine-Earth Science and Technology (JAMSTEC), ³Advanced Material Research Laboratory (AMRL), Clemson University, ⁴Swire Institute of Marine Sciences and School of Biological Sciences, The University of Hong Kong

Summary

We demonstrate the use of various microscopy methods that are useful in observing the calcification of a tubeworm, Hydroides elegans, as well as locating and characterizing the first calcified material. Live microscopy and electron microscopy are used together to provide functional and material information that are important in studying biomineralization.

Explore More Videos

Optical Microscopy

Electron Microscopy

Marine Tubeworm

Intracellular PH

Chapters in this video

0:00

Title

4:11

Screening for Life Stage and Tissue of Interest with Live Imaging

6:20

Sample Preservation, Dehydration and Mounting for Electron Microscopy

8:00

Location a Calcium Rich Region Using SEM-EDS

9:11

Identifying Crystallographic Information of Calcium Rich Regions Using SEM-EBSD

10:28

TEM Sample Preparation Using FIB-SEM

12:32

Obtaining Selected Area Diffraction Pattern on a TEM

13:47

Representative Results

15:05

Conclusion

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