JoVE Journal

Biology

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Nanyang Technological University

マリンTubewormでライブ光学および電子顕微鏡技術を用いた石灰化イベントのキャラクタリゼーション

We demonstrate the use of various microscopy methods that are useful in observing the calcification of a tubeworm, Hydroides elegans, as well as locating and characterizing the first calcified material. Live microscopy and electron microscopy are used together to provide functional and material information that are important in studying biomineralization.

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Title

4:11

Screening for Life Stage and Tissue of Interest with Live Imaging

6:20

Sample Preservation, Dehydration and Mounting for Electron Microscopy

8:00

Location a Calcium Rich Region Using SEM-EDS

9:11

Identifying Crystallographic Information of Calcium Rich Regions Using SEM-EBSD

10:28

TEM Sample Preparation Using FIB-SEM

12:32

Obtaining Selected Area Diffraction Pattern on a TEM

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Representative Results

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Conclusion

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