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Nanyang Technological University

Preparation of Prokaryotic and Eukaryotic Organisms Using Chemical Drying for Morphological Analysis in Scanning Electron Microscopy (SEM)

SEM analysis is an effective method to aid in species identification or phenotypic discrimination. This protocol describes the methods for examining specific morphological details of three representative types of organisms and would be broadly applicable to examining features of many organismal and tissue types.

Chapters in this video

0:04

Title

0:45

Fixation, Washing and Dehydration of Cyanobacteria and Euglenoids

2:36

Drying and Mounting

3:54

Fixation, Washing and Dehydration of Drosophila

4:55

Drosophila Drying and Mounting

6:48

Sputter Coating and Imaging

7:20

Results: Morphological Analysis of Different Organisms Using SEM Images

9:20

Conclusion

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