JoVE Journal
Biochemistry
You have full access to this content through
Nanyang Technological UniversityAn Optimized Protocol for Electrophoretic Mobility Shift Assay Using Infrared Fluorescent Dye-labeled Oligonucleotides
15.6K Views
•
09:58 min
•
November 29th, 2016
DOI :
November 29th, 2016
•Chapters in this video
0:05
Title
0:32
Preparation of Gel, Infrared Fluorescent Dye-labeled Probes and Unlabeled Probes
4:49
Binding Reaction and Electrophoresis
6:37
Imaging
7:35
Results: Electrophoretic Mobility Shift Assays Using Purified Proteins and Dye-labeled DNA Probes
8:57
Conclusion
Related Videos
06:36
Horizontal Gel Electrophoresis for Enhanced Detection of Protein-RNA Complexes
11.9K Views
07:38
DNA Polymerase Activity Assay Using Near-infrared Fluorescent Labeled DNA Visualized by Acrylamide Gel Electrophoresis
14.1K Views
04:18
DNA Electrophoresis Using Thiazole Orange Instead of Ethidium Bromide or Alternative Dyes
13.8K Views
05:33
Using Modified Synthetic Oligonucleotides to Assay Nucleic Acid-Metabolizing Enzymes
550 Views
04:07
Optical Photothermal Infrared-Fluorescence In Situ Hybridization (OPTIR-FISH)
1.2K Views
12:44
Electrophoretic Mobility Shift Assay (EMSA) for the Study of RNA-Protein Interactions: The IRE/IRP Example
53.6K Views
11:35
Screening for Functional Non-coding Genetic Variants Using Electrophoretic Mobility Shift Assay (EMSA) and DNA-affinity Precipitation Assay (DAPA)
12.9K Views
07:44
Synthesis of Wavelength-shifting DNA Hybridization Probes by Using Photostable Cyanine Dyes
11.1K Views
06:57
Electrophoretic Mobility Shift Assay (EMSA)
44.3K Views
01:51
Electrophoretic Mobility Shift Assay for Detection of Transcription Factor-DNA Complexes
353 Views
ISSN 2578-2037
Copyright © 2025 MyJoVE Corporation. All rights reserved
We use cookies to enhance your experience on our website.
By continuing to use our website or clicking “Continue”, you are agreeing to accept our cookies.