Horizontal Gel Electrophoresis for Enhanced Detection of Protein-RNA Complexes

11.9K Views

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06:36 min

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July 28th, 2017

DOI :

10.3791/56031-v

July 28th, 2017

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Megan E. Dowdle¹, Susanne Blaser Imboden¹, Sookhee Park¹, Sean P. Ryder², Michael D. Sheets¹

¹Department of Biomolecular Chemistry, University of Wisconsin School of Medicine and Public Health, ²Department of Biochemistry and Molecular Pharmacology, University of Massachusetts Medical School

Summary

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Keywords Horizontal Gel Electrophoresis

Protein RNA Complexes

RNA Biochemistry

RNA protein Interactions

Native Gel Electrophoresis

Fluorescently Labeled RNA

Bicaudal c Protein

Chapters in this video

0:05

Title

0:35

Preparing the Horizontal Native Polyacrylamide Gel

2:27

Binding Reaction

3:23

Analysis of Samples

4:12

Imaging the Gel

5:04

Results: Horizontal Gel Electrophoresis Enhances the Detection of Distinct RNA-protein Complexes

6:08

Conclusion

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