JoVE Journal

Bioengineering

Masz pełny dostęp do tej treści za pośrednictwem

Nanyang Technological University

Imaging-Guided Bioreactor for Generating Bioengineered Airway Tissue

Please note that all translations are automatically generated. Click here for the English version.

2.6K Views

•

11:01 min

•

April 6th, 2022

DOI :

10.3791/63544-v

April 6th, 2022

•

Mohammad Mir¹, Jiawen Chen¹, Meghan R. Pinezich¹, John D. O’Neill³, Brandon A. Guenthart⁴, Gordana Vunjak-Novakovic², Jinho Kim¹

¹Department of Biomedical Engineering, Stevens Institute of Technology, ²Department of Biomedical Engineering, Columbia University, ³Department of Cell Biology, State University of New York Downstate Medical Center, ⁴Department of Cardiothoracic Surgery, Stanford University

Podsumowanie

The protocol describes an imaging-enabled bioreactor that allows the selective removal of the endogenous epithelium from the rat trachea and homogenous distribution of exogenous cells on the lumen surface, followed by long-term in vitro culture of the cell-tissue construct.

Przeglądaj więcej filmów

Imaging guided Bioreactor

Bioengineered Airway Tissue

Airway Tissue Regeneration

Tissue Manipulation Protocols

In Situ Imaging

Tracheal Lumen Monitoring

Disease Modeling

Microscopy Setup

Bright field Imaging

Fluorescent Imaging

Scientific CMOS Camera

Optical Cage System

Rozdziały w tym wideo

0:05

Introduction

1:14

Construction of Gradient Index (GRIN) Lens-Based Imaging Device

2:25

Imaging-Guided Removal of the Trachea Epithelium

5:51

Homogeneous Distribution of Exogenous Cells onto the Denuded Tracheal Lumen

8:23

Results: Imaging-Guided Bioreactors for the Generation of Functional In Vitro Airway Tissues

10:18

Conclusion

Powiązane Filmy

article

Engineering Biological-Based Vascular Grafts Using a Pulsatile Bioreactor

17.0K Views

article

Procedure for Lung Engineering

46.6K Views

article

Nonhuman Primate Lung Decellularization and Recellularization Using a Specialized Large-organ Bioreactor

9.8K Views

article

Adapting the Electrospinning Process to Provide Three Unique Environments for a Tri-layered In Vitro Model of the Airway Wall

12.4K Views

article

Construction of Defined Human Engineered Cardiac Tissues to Study Mechanisms of Cardiac Cell Therapy

10.3K Views

article

A Multi-Cue Bioreactor to Evaluate the Inflammatory and Regenerative Capacity of Biomaterials under Flow and Stretch

5.5K Views

article

Generating 3D Spheres and 2D Air-Liquid Interface Cultures of Human Induced Pluripotent Stem Cell-Derived Type 2 Alveolar Epithelial Cells

4.7K Views

article

Generation of Airway Epithelial Cell Air-Liquid Interface Cultures from Human Pluripotent Stem Cells

4.1K Views

article

Seeding and Implantation of a Biosynthetic Tissue-engineered Tracheal Graft in a Mouse Model

7.2K Views

article

iPSC-Derived Epithelial, Mesenchymal, Endothelial, and Immune Cell Co-Culture to Model Airway Barrier Integrity in Lung Health and Disease

889 Views

Copyright © 2025 MyJoVE Corporation. Wszelkie prawa zastrzeżone

Używamy plików cookie, aby ulepszyć Twoje wrażenia z korzystania z naszej witryny.

Kontynuując korzystanie z naszej witryny lub klikając „Kontynuuj”, wyrażasz zgodę na akceptację naszych plików cookie.

Dowiedz się więcej