The Extraction of Liver Glycogen Molecules for Glycogen Structure Determination

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4.8K Views

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04:50 min

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February 8th, 2022

DOI :

10.3791/63088-v

February 8th, 2022

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Ziyi Wang¹^,², Qinghua Liu³^,⁴, Liang Wang³^,⁵, Robert G. Gilbert¹^,²^,⁶, Mitchell A. Sullivan⁷

¹School of Chemistry and Molecular Biosciences, The University of Queensland, ²Centre for Nutrition and Food Sciences, Queensland Alliance for Agriculture and Food Innovation, The University of Queensland, ³Jiangsu Provincial Key Laboratory of New Drug Research and Clinical Pharmacy, School of Pharmacy, Xuzhou Medical University, ⁴Department of Pharmaceutical Analysis, School of Pharmacy, Xuzhou Medical University, ⁵Department of Bioinformatics, School of Medical Informatics and Engineering, Xuzhou Medical University, ⁶Joint International Research Laboratory of Agriculture and Agri-Product Safety, College of Agriculture, Yangzhou University, ⁷Glycation and Diabetes Group, Mater Research Institute-The University of Queensland, Translational Research Institute

Podsumowanie

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Glycogen Extraction

Liver Glycogen

Glycogen Structure Determination

Diabetes

Glycogen Storage Disease

Glycogen Isolation Buffer

Tissue Homogenizer

Supernatant Centrifugation

Ethanol Precipitation

Glycogen Yield

Cryopreservation

Purity Analysis

Rozdziały w tym wideo

0:00

Introduction

0:30

Glycogen Extraction

2:44

Results: The Effect of Sucrose Concentration on the Particle Size of Glycogen

4:25

Conclusion

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