Production, Crystallization, and Structure Determination of the IKK-binding Domain of NEMO
Please note that all translations are automatically generated. Click here for the English version.
7.4K Views
•
13:02 min
•
December 28th, 2019
DOI :
December 28th, 2019
•Rozdziały w tym wideo
0:04
Title
1:12
Large Scale Expression of Histidine-6 (His6)-Tagged Nuclear Factor-kappa-B Essential Modulator Crystallography Construct (NEMO-EEAA)
2:33
His6-Tagged NEMO-EEAA Purification
4:42
His6 Tag Cleavage and Purification
7:09
Sparse Matrix Screening
8:23
Seed Stock Generation
9:00
Crystal Looping
9:43
X-Ray Data Processing and Structure Solution
10:38
Results: Representative Production, Crystallization, and Structure Determination of the IKK-Binding Domain of NEMO
12:28
Conclusion
Powiązane Filmy
11:14
Combining Wet and Dry Lab Techniques to Guide the Crystallization of Large Coiled-coil Containing Proteins
7.9K Views
11:27
A Guide to Production, Crystallization, and Structure Determination of Human IKK1/α
9.1K Views
09:55
From Constructs to Crystals – Towards Structure Determination of β-barrel Outer Membrane Proteins
13.4K Views
09:15
Combining X-Ray Crystallography with Small Angle X-Ray Scattering to Model Unstructured Regions of Nsa1 from S. Cerevisiae
9.8K Views
10:27
Contrast-Matching Detergent in Small-Angle Neutron Scattering Experiments for Membrane Protein Structural Analysis and Ab Initio Modeling
12.3K Views
07:19
Structural Studies of Macromolecules in Solution using Small Angle X-Ray Scattering
12.5K Views
11:31
Crystal Structure of the N-terminal Domain of Ryanodine Receptor from Plutella xylostella
7.4K Views
14:04
Derivatization of Protein Crystals with I3C using Random Microseed Matrix Screening
4.6K Views
06:46
Highly Efficient Transfection of Primary Macrophages with In Vitro Transcribed mRNA
22.2K Views
10:46
Production of Human Norovirus Protruding Domains in E. coli for X-ray Crystallography
8.6K Views
Copyright © 2025 MyJoVE Corporation. Wszelkie prawa zastrzeżone