Characterization of Neuromuscular Junctions in Mice by Combined Confocal and Super-Resolution Microscopy
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11:03 min
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December 8th, 2021
DOI :
December 8th, 2021
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Rozdziały w tym wideo
0:04
Introduction
1:17
Dissection of Muscles and Immunostaining
3:21
Image Acquisition
3:58
Image Analysis to Calculate Postsynaptic NMJ Endplate Volume, MIP Area, and Relative Tortuosity
5:05
Image Analysis to Quantify Presynaptic Neurofilament Accumulation and Synaptic Vesicle Glycoprotein 2 Staining
6:04
Calculating the Distance Between Acetylcholine Receptor (AChR) Stripes and Their Width
8:22
Results: Multi Parameter Postsynaptic NMJ Characterization, STED Imaging, and Morphometric Analysis of Presynaptic Axon Terminal Distribution
10:09
Conclusion
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