Extracellular Recording of Neuronal Activity Combined with Microiontophoretic Application of Neuroactive Substances in Awake Mice

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9.9K Views

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11:19 min

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May 21st, 2016

DOI :

10.3791/53914-v

May 21st, 2016

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Yaneri A. Ayala¹, David Pérez-González¹, Daniel Duque¹^,², Alan R. Palmer³, Manuel S. Malmierca¹^,⁴

¹Auditory Neuroscience Laboratory, Institute of Neuroscience of Castilla y León, University of Salamanca, ²Neural Systems Laboratory, Institute for Systems Research, University of Maryland, ³Medical Research Council Institute of Hearing Research, ⁴Department of Cell Biology and Pathology, Faculty of Medicine, University of Salamanca

Podsumowanie

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Keywords Extracellular Recording

Neuronal Activity

Microiontophoretic Application

Neuroactive Substances

Awake Mice

Sensory Systems

Neurotransmitters

Neuronal Modulatory Systems

Tungsten Wires

Etching

Borosilicate Glass Capillary

Boric Bead

Rozdziały w tym wideo

0:05

Title

0:54

Tungsten Electrodes

3:03

Multibarrel Glass Pipette Manufacturing

4:26

Surgery and Headpost Implantation

6:49

Electrophysiological Recording and Microiontophoresis

8:26

Results: Effect of the Blockade of the GABA_A-receptors on the Spectral and Temporal Response Properties as well as the Repetitive and Rare Sounds

9:58

Conclusion

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