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Neuroscience

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Nanyang Technological University

Protocol for Three-dimensional Confocal Morphometric Analysis of Astrocytes

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8.8K Views

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09:13 min

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December 11th, 2015

DOI :

10.3791/53113-v

December 11th, 2015

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Maryam Bagheri¹, Arjang Rezakhani¹, Mehrdad Roghani², Mohammad T. Joghataei³^,⁴, Simin Mohseni¹

¹Department of Clinical and Experimental Medicine, Linköping University, ²Neurophysiology Research Center, Shahed University, ³Cellular and Molecular Research Center, Iran University of Medical Sciences, ⁴School of Advanced Technologies in Medicine, Iran University of Medical Sciences

Podsumowanie

Astrocytes in the CNS change their functional and structural properties in response to harmful stimuli. This report presents a protocol for assessment of three-dimensional astrocyte morphology in diseased conditions or after therapeutic interventions.

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Keywords 3D Confocal Microscopy

Astrocyte Morphology

Astrocyte Territory

Astrocyte Volume

Astrocyte Density

Reactive Astrogliosis

Volocity Software

Rozdziały w tym wideo

0:05

Title

0:46

Immunohistochemistry of Rat Brain Slices

2:38

Confocal Microscopy

4:51

Data Analysis of 2D and 3D Confocal Images

7:17

Results: Qualitative and Quantitative Analysis of Astrogliosis

8:19

Conclusion

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