JoVE Journal

Biochemistry

This content is Open Access.

Single Particle Cryo-Electron Microscopy: From Sample to Structure

Please note that all translations are automatically generated. Click here for the English version.

8.3K Views

•

11:52 min

•

May 29th, 2021

DOI :

10.3791/62415-v

May 29th, 2021

•

Joshua B.R. White¹, Daniel P. Maskell¹, Andrew Howe², Martin Harrow², Daniel K. Clare², C. Alistair Siebert², Emma L. Hesketh¹, Rebecca F. Thompson¹

¹Astbury Centre Structural Molecular Biology, School Molecular and Cellular Biology, Faulty Biological Sciences, University of Leeds, ²Diamond Light Source, Harwell Science and Innovation Campus

Podsumowanie

Structure determination of macromolecular complexes using cryoEM has become routine for certain classes of proteins and complexes. Here, this pipeline is summarized (sample preparation, screening, data acquisition and processing) and readers are directed towards further detailed resources and variables that may be altered in the case of more challenging specimens.

Przeglądaj więcej filmów

Single Particle Cryo Electron Microscopy

Structural Biology

Sample Preparation

Cryo EM Facilities

Astbury Biostructure Laboratory

Remote Data Collection

Acquisition Optics

Sample Quality Assessment

Imaging Techniques

Rozdziały w tym wideo

0:04

Introduction

0:47

Performing an Inventory and Screening for Ice and Sample Quality

4:07

Single Particle CryoEM Data Collection

9:44

Results: Single Particle Cryo-Electron Microscopy: Screening for 3D Classification and Refinement

11:04

Conclusion

Powiązane Filmy

article

Cryo-EM and Single-Particle Analysis with Scipion

3.7K Views

article

Preparation and Cryo-FIB micromachining of Saccharomyces cerevisiae for Cryo-Electron Tomography

4.2K Views

article

Manual Blot-and-Plunge Freezing of Biological Specimens for Single-Particle Cryogenic Electron Microscopy

6.2K Views

article

A Robust Single-Particle Cryo-Electron Microscopy (cryo-EM) Processing Workflow with cryoSPARC, RELION, and Scipion

12.9K Views

article

Routine Collection of High-Resolution cryo-EM Datasets Using 200 KV Transmission Electron Microscope

5.0K Views

article

Single-Particle Cryo-EM Data Collection with Stage Tilt using Leginon

2.3K Views

article

Optimizing Sample Preparation for Cryogenic Electron Microscopy

234 Views

article

Do's and Don'ts of Cryo-electron Microscopy: A Primer on Sample Preparation and High Quality Data Collection for Macromolecular 3D Reconstruction

46.0K Views

article

Cryo-Electron Tomography Remote Data Collection and Subtomogram Averaging

4.7K Views

article

Tomografia mikroskopu elektronowego i rekonstrukcja pojedynczych cząstek

2.3K Views

Copyright © 2025 MyJoVE Corporation. Wszelkie prawa zastrzeżone

Używamy plików cookie, aby ulepszyć Twoje wrażenia z korzystania z naszej witryny.

Kontynuując korzystanie z naszej witryny lub klikając „Kontynuuj”, wyrażasz zgodę na akceptację naszych plików cookie.

Dowiedz się więcej